The selective expression of carbonic anhydrase genes of Aspergillus nidulans in response to changes in mineral nutrition and CO2 concentration

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	The selective expression of carbonic anhydrase genes of Aspergillus nidulans in response to changes in mineral nutrition and CO2 concentration
	Xiao, LL ; Lian, B ; Dong, CL ; Liu, FH
刊名	MICROBIOLOGYOPEN
	2016-02-01
卷号	5 期号:1 页码:60-69
关键词	Aspergillus nidulans carbonic anhydrase CO2 adaption isoenzyme mineral weathering
ISSN号	2045-8827
通讯作者	Lian, B (reprint author), Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Microbes & Funct Genom, Jiangsu Engn & Technol Res Ctr Microbiol, Nanjing 210023, Jiangsu, Peoples R China. bin2368@vip.163.com
产权排序	[Xiao, Leilei; Lian, Bin; Dong, Cuiling] Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Microbes & Funct Genom, Jiangsu Engn & Technol Res Ctr Microbiol, Nanjing 210023, Jiangsu, Peoples R China; [Xiao, Leilei; Liu, Fanghua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
英文摘要	Carbonic anhydrase (CA) plays an important role in the formation and evolution of life. However, to our knowledge, there has been no report on CA isoenzyme function differentiation in fungi. Two different CA gene sequences in Aspergillus nidulans with clear genetic background provide us a favorable basis for studying function differentiation of CA isoenzymes. Heterologously expressed CA1 was used to test its weathering ability on silicate minerals and real-time quantitative PCR was used to detect expression of the CA1 and CA2 genes at different CO2 concentrations and in the presence of different potassium sources. The northern blot method was applied to confirm the result of CA1 gene expression. Heterologously expressed CA1 significantly promoted dissolution of biotite and wollastonite, and CA1 gene expression increased significantly in response to soluble K-deficiency. The northern blot test further showed that CA1 participated in K-feldspar weathering. In addition, the results showed that CA2 was primary involved in adapting to CO2 concentration change. Taken together, A. nidulans can choose different CA to meet their survival needs, which imply that some environmental microbes have evolved different CAs to adapt to changes in CO2 concentration and acquire mineral nutrition so that they can better adapt to environmental changes. Inversely, their adaption may impact mineral weathering and/or CO2 concentration, and even global change.
研究领域[WOS]	Microbiology
关键词[WOS]	CHLAMYDOMONAS-REINHARDTII ; ESCHERICHIA-COLI ; EVOLUTION ; DIOXIDE ; DISSOLUTION ; MECHANISMS ; DIVERSITY ; MICROORGANISMS ; NEOFORMANS ; FUMIGATUS
收录类别	SCI
语种	英语
WOS记录号	WOS:000371259500005
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/10837]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Microbes & Funct Genom, Jiangsu Engn & Technol Res Ctr Microbiol 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat
推荐引用方式 GB/T 7714	Xiao, LL,Lian, B,Dong, CL,et al. The selective expression of carbonic anhydrase genes of Aspergillus nidulans in response to changes in mineral nutrition and CO2 concentration[J]. MICROBIOLOGYOPEN,2016,5(1):60-69.
APA	Xiao, LL,Lian, B,Dong, CL,&Liu, FH.(2016).The selective expression of carbonic anhydrase genes of Aspergillus nidulans in response to changes in mineral nutrition and CO2 concentration.MICROBIOLOGYOPEN,5(1),60-69.
MLA	Xiao, LL,et al."The selective expression of carbonic anhydrase genes of Aspergillus nidulans in response to changes in mineral nutrition and CO2 concentration".MICROBIOLOGYOPEN 5.1(2016):60-69.