Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe

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	Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe
	Ji, Wei ; Du, Lingyun ; Zhang, Yuanfu ; Liu, Guofu ; Wang, Shuhao
刊名	FOOD ANALYTICAL METHODS
	2015
卷号	8 期号:10 页码:2596-2604
关键词	LINKED-IMMUNOSORBENT-ASSAY TANDEM MASS-SPECTROMETRY HUMAN EXPOSURE MIGRATION FOOD BPA SAMPLES DNA TEMPERATURE BIOSENSOR
ISSN号	1936-9751
中文摘要	An ultrasensitive fluorescence immunoassay was developed for detection of bisphenol A (BPA). Herein, thiol-modified single-strand DNA (SH-ssDNAs) and anti-BPA antibodies were simultaneously conjugated with gold nanoparticles (AuNPs), and the fluorescein isothiocyanate-labeled single-strand DNA was hybridized with SH-ssDNAs to form a dual-codified probe. In the presence of BPA, a competitive immunoreaction was conducted between BPA and BPA coating antigens immobilized on microplate for the anti-BPA antibodies of dual-codified probe. Then, in order to avoid the fluorescent signals quenched by AuNPs, restriction enzyme BamH D dagger was employed to cut the fluorescent labels from immunocomplex, resulting in an increase of fluorescence emission. Under optimized conditions, the linear range for detection of BPA is from 1.0 x 10(-2) to 1.0 x 10(3) ng/L with a low limit of detection of 3.4 x 10(-3) ng/L. This method is of good sensibility, stability, and specificity, and it can be used to detect BPA in actual milk products and its migration from packaging materials into milk.
收录类别	SCI
原文出处	10.1007/s12161-015-0162-2
语种	英语
公开日期	2016-05-12
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/9803]
专题	西双版纳热带植物园_文献共享
推荐引用方式 GB/T 7714	Ji, Wei,Du, Lingyun,Zhang, Yuanfu,et al. Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe[J]. FOOD ANALYTICAL METHODS,2015,8(10):2596-2604.
APA	Ji, Wei,Du, Lingyun,Zhang, Yuanfu,Liu, Guofu,&Wang, Shuhao.(2015).Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe.FOOD ANALYTICAL METHODS,8(10),2596-2604.
MLA	Ji, Wei,et al."Ultrasensitive Fluorescence Immunoassay for Detection of Bisphenol A in Milk Products Using Functionalized Gold Nanoparticles as Probe".FOOD ANALYTICAL METHODS 8.10(2015):2596-2604.