Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2

	Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2
	Wan, Chanjuan 1; Wu, Bo 1; Song, Zhenwei 2,3; Zhang, Jiahai 2,3; Chu, Huiying 4; Wang, Aoli 1; Liu, Qingsong 1; Shi, Yunyu 2,3; Li, Guohui 4; Wang, Junfeng 1
刊名	chemistry and physics of lipids
	2015-02-01
卷号	186 页码:61-67
关键词	NMR Granuphilin C2 domain PI(4 Nanodisc Recognition mechanism 5)P2
英文摘要	phosphatidylinositol 4,5-bisphosphate (pi(4,5)p2) is a key player in regulating the process of excytosis, including insulin secretion. granuphilin, a tandem c2 domain containing protein, mediates the docking of insulin granules onto plasma membrane. the c2a domain plays key roles in this process through interaction with pi(4,5)p2. in this study, we have investigated the molecular recognition mechanism of granuphilin-c2a domain to pi(4,5)p2 head group, and further to pi(4,5)p2-nanodisc by nmr, itc, mst and sec methods. our results demonstrate that pi(4,5)p2 binds to the concave surface of granuphilin-c2a domain. the key residues involved in the binding were validated by mutation analysis. (c) 2015 elsevier ireland ltd. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; biophysics
研究领域[WOS]	biochemistry & molecular biology ; biophysics
关键词[WOS]	exocytosis ; granules ; complex
收录类别	SCI
语种	英语
WOS记录号	WOS:000352051200008
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/146155]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, High Field Magnet Lab, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Heifei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wan, Chanjuan,Wu, Bo,Song, Zhenwei,et al. Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2[J]. chemistry and physics of lipids,2015,186:61-67.
APA	Wan, Chanjuan.,Wu, Bo.,Song, Zhenwei.,Zhang, Jiahai.,Chu, Huiying.,...&Wang, Junfeng.(2015).Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2.chemistry and physics of lipids,186,61-67.
MLA	Wan, Chanjuan,et al."Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2".chemistry and physics of lipids 186(2015):61-67.