Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix

	Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix
	Zhao Yanyan 1; Wang Liheng 2; Guo Zhimou 3; Chi Xiaofei 2; Ma Xiaochi 1; Qi Yan 1; Fang Shinong 1; Li Xiuling 3; Liang Xinmiao 3
刊名	chemical research in chinese universities
	2015-02-01
卷号	31 期号:1 页码:44-52
关键词	Click OEG-CD[olio(ethylene glycol)(OEG) linked beta-cyclodextrin(CD)] matrix Multi-phosphopeptide enrichment MS detection
英文摘要	detection and determination of multi-phosphopeptides from protein digestion products are difficult due to the low abundance and ion-suppression effect arised from the existence of their high-abundance counterparts. click oeg-cd[olio(ethylene glycol)(oeg) linked,beta-cyclodextrin(cd)] matrix has shown excellent performance in phosphopeptide enrichment. however, few multi-phosphopeptides were detected previously. in this investigation, an improved method aiming at enhancing the enrichment selectivity and mass spectrometry(ms) detection of multi-phosphopeptide was developed via optimizing the sample loading amount on per mg of matrix. mass spectra were obtained on a nano liquid chromatography-electrospray ionization-quadrupole time of flight-mass spectrometry (lc-esi-qtoe-ms). the enrichment selectivity of double-phosphopeptide could be enhanced with the increase of loading amount on per mg of matrix when taking the mixture of mono-, double-, and non-phosphopeptides as probe. furthermore, the multi-phosphopeptide enrichment selectivity was enhanced under the condition of optimized loading amount when taking the product of typtic digestion a-casein as test sample. when the loading amount was 573 pmol/mg matrix, up to 20 a-casein phosphopeptide signals(including 15 multi-phosphopeptides) were detected. the result is much better than that of our previous report. the reduction of ion-suppression effect arised from the existence of high-abundance non- or mono-phosphopeptides and the stronger interactions between multi-phosphopepides and the matrix were contributed to the result. the study could be helpful to the better utilization of click oeg,cd matrix in the enrichment of multi-phosphopeptide from complex biosamples in the subsequent investigation.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	phosphoproteome analysis ; phosphorylation analysis ; protein-phosphorylation ; selective enrichment ; chromatography ; peptides
收录类别	SCI
语种	英语
WOS记录号	WOS:000351479100009
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/146104]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China 2.Second Hosp Dalian City, Dalian 116011, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhao Yanyan,Wang Liheng,Guo Zhimou,et al. Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix[J]. chemical research in chinese universities,2015,31(1):44-52.
APA	Zhao Yanyan.,Wang Liheng.,Guo Zhimou.,Chi Xiaofei.,Ma Xiaochi.,...&Liang Xinmiao.(2015).Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix.chemical research in chinese universities,31(1),44-52.
MLA	Zhao Yanyan,et al."Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOE-MS Detection Strategy Using Click OEG-CD Matrix".chemical research in chinese universities 31.1(2015):44-52.