Effects of Short-Chain Chlorinated Paraffins Exposure on the Viability and Metabolism of Human Hepatoma HepG2 Cells

	Effects of Short-Chain Chlorinated Paraffins Exposure on the Viability and Metabolism of Human Hepatoma HepG2 Cells
	Geng, Ningbo 1,2; Zhang, Haijun 1; Zhang, Baoqin 1; Wu, Ping 1; Wang, Feidi 1,2; Yu, Zhengkun 1; Chen, Jiping 1
刊名	environmental science & technology
	2015-03-03
卷号	49 期号:5 页码:3076-3083
英文摘要	short-chain chlorinated paraffins (sccps) have attracted considerable attention for their characteristic of persistent organic pollutants. however, very limited information is available for their toxic effects at environmentally relevant doses, limiting the evaluation of their health risks. in this study, cell viability assay and targeted metabolomic approach was used to evaluate the environmental dose (<100 mu g/l) effect of sccps on hepg2 cells. cell viability was found to be decreased with increases in exposure dose of sccps. exposure for 48 h to c-10-cps resulted in a significant reduction in cell viability compared with 24 h, even at 1 mu g/l. sccps exposure altered the intracellular redox status and caused significant metabolic disruptions. as a kind of peroxisome proliferator, sccps specifically stimulated the beta-oxidation of unsaturated fatty acids and long-chain fatty acids. meanwhile, sccps exposure disturbed glycolysis and amino acid metabolism, and led to the up-regulation of glutamate metabolism and urea cycle. the toxic effects of sccps might mainly involve the perturbation of energy production, protein biosynthesis, fatty acid metabolism, and ammonia recycling.
WOS标题词	science & technology ; technology ; life sciences & biomedicine
类目[WOS]	engineering, environmental ; environmental sciences
研究领域[WOS]	engineering ; environmental sciences & ecology
关键词[WOS]	polychloro-n-alkanes ; environmental occurrence ; sample preparation ; metabolomics ; c-10-c-13 ; toxicity ; liver ; rats ; sediments ; responses
收录类别	SCI
语种	英语
WOS记录号	WOS:000350611100062
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/146080]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Geng, Ningbo,Zhang, Haijun,Zhang, Baoqin,et al. Effects of Short-Chain Chlorinated Paraffins Exposure on the Viability and Metabolism of Human Hepatoma HepG2 Cells[J]. environmental science & technology,2015,49(5):3076-3083.
APA	Geng, Ningbo.,Zhang, Haijun.,Zhang, Baoqin.,Wu, Ping.,Wang, Feidi.,...&Chen, Jiping.(2015).Effects of Short-Chain Chlorinated Paraffins Exposure on the Viability and Metabolism of Human Hepatoma HepG2 Cells.environmental science & technology,49(5),3076-3083.
MLA	Geng, Ningbo,et al."Effects of Short-Chain Chlorinated Paraffins Exposure on the Viability and Metabolism of Human Hepatoma HepG2 Cells".environmental science & technology 49.5(2015):3076-3083.