High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside

	High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside
	Liu, Jing 1,2; Wang, Fangjun 1; Mao, Jiawei 1,2; Zhang, Zhang 1,2; Liu, Zheyi 1,2; Huang, Guang 1,2; Cheng, Kai 1,2; Zou, Hanfa 1
刊名	analytical chemistry
	2015-02-17
卷号	87 期号:4 页码:2054-2057
英文摘要	n-dodecyl beta-d-maltoside (ddm), a mild detergent with the ability to maintain the enzyme activity and solubilize hydrophobic proteins without changing their structures, was applied for n-glycoproteomic analysis of minute protein sample from mouse brain tissue. after combining with the capillary-based glycoproteomic reactor, 281 n-glycosylation sites were successfully characterized from 50 mu g of mouse brain tissue, which was 110% higher at least than those obtained by conventional strategies.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	spectrometry-compatible surfactants ; sample preparation method ; mass-spectrometry ; membrane-proteins ; cells ; proteomics ; glycosylation ; purification
收录类别	SCI
语种	英语
WOS记录号	WOS:000349806200007
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145932]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jing,Wang, Fangjun,Mao, Jiawei,et al. High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside[J]. analytical chemistry,2015,87(4):2054-2057.
APA	Liu, Jing.,Wang, Fangjun.,Mao, Jiawei.,Zhang, Zhang.,Liu, Zheyi.,...&Zou, Hanfa.(2015).High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside.analytical chemistry,87(4),2054-2057.
MLA	Liu, Jing,et al."High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside".analytical chemistry 87.4(2015):2054-2057.