High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside | |
Liu, Jing1,2; Wang, Fangjun1; Mao, Jiawei1,2; Zhang, Zhang1,2; Liu, Zheyi1,2; Huang, Guang1,2; Cheng, Kai1,2; Zou, Hanfa1 | |
刊名 | analytical chemistry
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2015-02-17 | |
卷号 | 87期号:4页码:2054-2057 |
英文摘要 | n-dodecyl beta-d-maltoside (ddm), a mild detergent with the ability to maintain the enzyme activity and solubilize hydrophobic proteins without changing their structures, was applied for n-glycoproteomic analysis of minute protein sample from mouse brain tissue. after combining with the capillary-based glycoproteomic reactor, 281 n-glycosylation sites were successfully characterized from 50 mu g of mouse brain tissue, which was 110% higher at least than those obtained by conventional strategies. |
WOS标题词 | science & technology ; physical sciences |
类目[WOS] | chemistry, analytical |
研究领域[WOS] | chemistry |
关键词[WOS] | spectrometry-compatible surfactants ; sample preparation method ; mass-spectrometry ; membrane-proteins ; cells ; proteomics ; glycosylation ; purification |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000349806200007 |
公开日期 | 2016-05-09 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145932] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Jing,Wang, Fangjun,Mao, Jiawei,et al. High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside[J]. analytical chemistry,2015,87(4):2054-2057. |
APA | Liu, Jing.,Wang, Fangjun.,Mao, Jiawei.,Zhang, Zhang.,Liu, Zheyi.,...&Zou, Hanfa.(2015).High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside.analytical chemistry,87(4),2054-2057. |
MLA | Liu, Jing,et al."High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl beta-D-Maltoside".analytical chemistry 87.4(2015):2054-2057. |
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