Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs

	Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs
	Hou, Shuhua 1,2,3; Ji, Debin 1,2; Liu, Wujun 1,2; Wang, Lei 1,2; Zhao, Zongbao (Kent)1,2
刊名	bioorganic & medicinal chemistry letters
	2014-03-01
卷号	24 期号:5 页码:1307-1309
关键词	Cofactor Malic enzyme Nicotinamide adenine dinucleotide Orthogonal redox system Enzyme kinetics
英文摘要	an activity screening between 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide (nad) analogs and malic enzyme (me) mutants identified some mutants capable of taking nad analogs as the cofactor. one particular pair, me-l310k/l404s and the analog b-8 had good catalytic efficiency and cofactor specificity. the new system gained about 1200-fold cofactor specificity shift from nad toward b-8 in terms of oxidative decarboxylation of l-malate. our results provided insightful information for the development of orthogonal redox system that is of particular important to precisely control engineered metabolic pathways. (c) 2014 elsevier ltd. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	chemistry, medicinal ; chemistry, organic
研究领域[WOS]	pharmacology & pharmacy ; chemistry
关键词[WOS]	bioorthogonal chemistry ; small molecules ; nad analogs ; discrimination ; dehydrogenase ; specificity ; systems ; life
收录类别	SCI
语种	英语
WOS记录号	WOS:000331705200012
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145771]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Div Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, State Key Lab Catalysis, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Bohai Univ, Dept Chem, Liaoning Key Lab Synth & Applicat Funct Cpds, Jinzhou 121013, Peoples R China
推荐引用方式 GB/T 7714	Hou, Shuhua,Ji, Debin,Liu, Wujun,et al. Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs[J]. bioorganic & medicinal chemistry letters,2014,24(5):1307-1309.
APA	Hou, Shuhua,Ji, Debin,Liu, Wujun,Wang, Lei,&Zhao, Zongbao .(2014).Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs.bioorganic & medicinal chemistry letters,24(5),1307-1309.
MLA	Hou, Shuhua,et al."Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs".bioorganic & medicinal chemistry letters 24.5(2014):1307-1309.