Selective Extraction of Low Molecular Weight Proteins by Mesoporous Silica Particles with Phenyl Modified Internal and Alkyl-diol Modified External Surfaces

	Selective Extraction of Low Molecular Weight Proteins by Mesoporous Silica Particles with Phenyl Modified Internal and Alkyl-diol Modified External Surfaces
	Qi Yan-Xia 1,2; Zhao Qian-Cheng 1; Li Zhi-Bo 1; Li Wei 1; Wang Qiu-Kuan 1; Guan Ya-Feng 2
刊名	chinese journal of analytical chemistry
	2014-08-01
卷号	42 期号:8 页码:1083-1087
关键词	Mesoporous silica Internal and external surfaces modification Selective extraction Low molecular weight protein Biological sample preparation
英文摘要	selective extraction of low molecular weight (lmw) proteins and peptides from complex biological samples plays an important role in the discovery of useful biomarkers and signaling molecules. it was demonstrated that the unique pore structure of mesoporous material made it efficient to enrich lmw proteins and peptides from complex matrixes. in this study, a mesoporous material with modified external (alkyl-diol group) and internal (phenyl group) surfaces (alkyl-diol@phenyl-sio2) was prepared by co-condensation and post-grafting, and its characteristic was evaluated by fourier transform-infrared spectra (ft-ir) and mass spectrometry (ms). the lmw proteins and peptides enriched by the alkyl-diol@phenyl-sio2 mesoporous material could be easily eluted by organic solvents, which was compatible with the following detection by ms. this new mesoporous material exhibited good selectivity for the extraction of lmw proteins and peptides (less than 10 kda) from complex biological samples.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	liquid-chromatography ; separation ; functionalization ; enrichment ; peptides ; column ; serum
收录类别	SCI
语种	英语
WOS记录号	WOS:000342387200003
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145742]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Ocean Univ, Coll Food Sci & Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Qi Yan-Xia,Zhao Qian-Cheng,Li Zhi-Bo,et al. Selective Extraction of Low Molecular Weight Proteins by Mesoporous Silica Particles with Phenyl Modified Internal and Alkyl-diol Modified External Surfaces[J]. chinese journal of analytical chemistry,2014,42(8):1083-1087.
APA	Qi Yan-Xia,Zhao Qian-Cheng,Li Zhi-Bo,Li Wei,Wang Qiu-Kuan,&Guan Ya-Feng.(2014).Selective Extraction of Low Molecular Weight Proteins by Mesoporous Silica Particles with Phenyl Modified Internal and Alkyl-diol Modified External Surfaces.chinese journal of analytical chemistry,42(8),1083-1087.
MLA	Qi Yan-Xia,et al."Selective Extraction of Low Molecular Weight Proteins by Mesoporous Silica Particles with Phenyl Modified Internal and Alkyl-diol Modified External Surfaces".chinese journal of analytical chemistry 42.8(2014):1083-1087.