Highly efficient N-glycoproteomic sample preparation by combining C-18 and graphitized carbon adsorbents

	Highly efficient N-glycoproteomic sample preparation by combining C-18 and graphitized carbon adsorbents
	Liu, Jing 1,2; Wang, Fangjun 1; Zhu, Jun 1,2; Mao, Jiawei 1,2; Liu, Zheyi 1,2; Cheng, Kai 1,2; Qin, Hongqiang 1; Zou, Hanfa 1
刊名	analytical and bioanalytical chemistry
	2014-05-01
卷号	406 期号:13 页码:3103-3109
关键词	Porous graphitized carbon C-18 adsorbent N-glycoproteomic sample preparation Proteomics LC-MS/MS
英文摘要	conventional n-glycoproteome analysis usually applies c-18 reversed-phase (rp) adsorbent for sample purification, which will lead to unavoidable sample loss due to the high hydrophilicity of n-glycopeptides. in this study, a porous graphitized carbon (pgc) absorbent was combined with a c-18 adsorbent for n-glycopeptide purification in comprehensive n-glycoproteome analysis based on the hydrophobic and polar interactions between carbon and n-glycans. it was observed that the small hydrophilic n-glycopeptides that cannot retain onto c-18 adsorbent can be captured by the graphitized carbon, while the large hydrophobic n-glycopeptides that cannot retain onto the graphitized carbon can be feasibly captured by the c-18 adsorbent. comparing with sample purification by using c-18 adsorbent only, 28.5 % more n-glycopeptides were identified by combining both c-18 and pgc adsorbents. the c-18-pgc strategy was further applied for both sample purification and pre-fractionation of a complex protein sample from hela cell. after hydrophilic interaction chromatography enrichment, 1,484 unique n-glycopeptides with 1,759 unique n-glycosylation sites were finally identified.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	liquid-chromatography ; glycans ; phase ; oligosaccharides ; glycopeptides ; glycosylation ; proteomics ; peptides ; protein ; cancer
收录类别	SCI
语种	英语
WOS记录号	WOS:000334685400011
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145575]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jing,Wang, Fangjun,Zhu, Jun,et al. Highly efficient N-glycoproteomic sample preparation by combining C-18 and graphitized carbon adsorbents[J]. analytical and bioanalytical chemistry,2014,406(13):3103-3109.
APA	Liu, Jing.,Wang, Fangjun.,Zhu, Jun.,Mao, Jiawei.,Liu, Zheyi.,...&Zou, Hanfa.(2014).Highly efficient N-glycoproteomic sample preparation by combining C-18 and graphitized carbon adsorbents.analytical and bioanalytical chemistry,406(13),3103-3109.
MLA	Liu, Jing,et al."Highly efficient N-glycoproteomic sample preparation by combining C-18 and graphitized carbon adsorbents".analytical and bioanalytical chemistry 406.13(2014):3103-3109.