Marine phytoplankton motility sensor integrated into a microfluidic chip for high-throughput pollutant toxicity assessment

	Marine phytoplankton motility sensor integrated into a microfluidic chip for high-throughput pollutant toxicity assessment
	Zheng, Guo-xia 1,3; Li, Ya-jie 2,3; Qi, Lin-lin 1,2; Liu, Xian-ming 3,4; Wang, Hu 1,3; Yu, Shu-ping 1,3; Wang, Yun-hua 2,3
刊名	marine pollution bulletin
	2014-07-15
卷号	84 期号:1-2 页码:147-154
关键词	Microfluidic chip Marine phytoplankton Motility Toxicity assessment High-throughput
英文摘要	a microfluidic chip was designed to assess the toxicity of pollutants in a high-throughput way by using marine phytoplankton motility as a sensor signal. in this chip, multiple gradient generators (cggs) with diffusible chambers enable large scale of dose-response bioassays to be performed in a simple way. two mobile marine phytoplankton cells were confined on-chip and stimulated by 8 concentrations (generated by cgg) of hg, pb, cu and phenol singly, as well as cu and phenol jointly. casa system was used to characterize motility by motile percentage (%mot), curvilinear velocity (vcl), average path velocity (vap) and straight line velocity (vsl). in all cases, dose-dependent inhibitions of motility were observed. in the present system, only 2 h was needed to predict ec50. thus, the developed microfluidic chip device was proved to be useful as a rapid/simple and high-throughput test method in marine pollution toxicity assessment. (c) 2014 elsevier ltd. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	environmental sciences ; marine & freshwater biology
研究领域[WOS]	environmental sciences & ecology ; marine & freshwater biology
关键词[WOS]	sperm motility ; daphnia-magna ; heavy-metals ; algae ; copper ; chlamydomonas ; microalgae ; chemicals ; bacteria ; cadmium
收录类别	SCI
语种	英语
WOS记录号	WOS:000338804700030
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145572]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Univ, Chem & Environm Engn Inst, Dalian 116012, Peoples R China 2.Dalian Univ, Sch Med, Dalian 116012, Peoples R China 3.Dalian Univ, Microfluid Res Inst, Dalian 116012, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Guo-xia,Li, Ya-jie,Qi, Lin-lin,et al. Marine phytoplankton motility sensor integrated into a microfluidic chip for high-throughput pollutant toxicity assessment[J]. marine pollution bulletin,2014,84(1-2):147-154.
APA	Zheng, Guo-xia.,Li, Ya-jie.,Qi, Lin-lin.,Liu, Xian-ming.,Wang, Hu.,...&Wang, Yun-hua.(2014).Marine phytoplankton motility sensor integrated into a microfluidic chip for high-throughput pollutant toxicity assessment.marine pollution bulletin,84(1-2),147-154.
MLA	Zheng, Guo-xia,et al."Marine phytoplankton motility sensor integrated into a microfluidic chip for high-throughput pollutant toxicity assessment".marine pollution bulletin 84.1-2(2014):147-154.