Serum Metabolomics Study of the Acute Graft Rejection in Human Renal Transplantation Based on Liquid Chromatography-Mass Spectrometry

	Serum Metabolomics Study of the Acute Graft Rejection in Human Renal Transplantation Based on Liquid Chromatography-Mass Spectrometry
	Zhao, Xinjie 1; Chen, Jihong 2; Ye, Lei 2; Xu, Guowang 1
刊名	journal of proteome research
	2014-05-01
卷号	13 期号:5 页码:2659-2667
关键词	metabolomics HILIC RPLC acute graft rejection renal transplantation
英文摘要	acute graft rejection is one of the most common and serious postcomplications in renal transplantation. a noninvasive method is needed to specifically monitor acute graft rejection. we investigated metabolic alterations of acute graft rejection in human renal transplantation by applying a metabolomics approach. sera from 11 acute graft rejection subjects and 16 nonacute graft rejection subjects were analyzed by a nontargeted liquid chromatography mass spectrometry (lc-ms) metabolomics approach including both hydrophilic interaction chromatography and reversed-phase liquid chromatography separations. discriminative metabolites of acute graft rejection after transplantation were detected, including creatinine, kynurenine, uric acid, polyunsaturated fatty acid, phosphatidylcholines, sphingomyelins, lysophosphatidylcholines, etc. the lower level of serum dehydroepiandrosterone sulfate was found in the acute graft rejection group before transplantation. the results revealed comprehensive metabolic abnormalities in acute graft rejection. the findings are valuable for the clinic noninvasive diagnosis or therapy of acute graft rejection.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	indoleamine 2,3-dioxygenase ; kidney-transplants ; metabonomic analysis ; cell proliferation ; phospholipase a(2) ; xanthine-oxidase ; immune function ; cyclosporine-a ; bile-acids ; t-cell
收录类别	SCI
语种	英语
WOS记录号	WOS:000335490600035
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145504]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Xinjiang Med Univ, Hosp 1, Dept Nephrol, Urumqi 830054, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xinjie,Chen, Jihong,Ye, Lei,et al. Serum Metabolomics Study of the Acute Graft Rejection in Human Renal Transplantation Based on Liquid Chromatography-Mass Spectrometry[J]. journal of proteome research,2014,13(5):2659-2667.
APA	Zhao, Xinjie,Chen, Jihong,Ye, Lei,&Xu, Guowang.(2014).Serum Metabolomics Study of the Acute Graft Rejection in Human Renal Transplantation Based on Liquid Chromatography-Mass Spectrometry.journal of proteome research,13(5),2659-2667.
MLA	Zhao, Xinjie,et al."Serum Metabolomics Study of the Acute Graft Rejection in Human Renal Transplantation Based on Liquid Chromatography-Mass Spectrometry".journal of proteome research 13.5(2014):2659-2667.