A novel method for characterization and comparison of reversed-phase column selectivity

	A novel method for characterization and comparison of reversed-phase column selectivity
	Wang, Jixia; Wang, Chaoran; Guo, Zhimou; Dong, Xuefang; Xiao, Yuansheng; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao
刊名	journal of chromatography a
	2014-09-26
卷号	1361 页码:153-161
关键词	Reversed-phase column selectivity LSERs Fundamental retention equations CSASS Three linear gradient elutions
英文摘要	characterization of reverse-phase column selectivity is helpful for chromatographers to select an optimal column. a novel method, based on linear solvation energy relationships (lsers) combined with fundamental retention equations, was developed to characterize and compare reversed-phase column selectivity. the retention times of 25 elaborately selected solutes on 12 reversed-phase columns were determined in three linear gradient elutions. using these retention times, fundamental retention equations were acquired by a complex sample analysis software system (csass). when 0%, 10%, 20%, 30%, 40% and 50% acetonitrile were introduced into the fundamental retention equations, the corresponding retention factors were predicted and used to obtain lser equations by multiple linear regression. in the gradient elution, the retention times of solutes could be accurately determined and the excessively long or short analysis time could be avoided. as the retention factor (ln k(w)) at a hypothetical 0% organic modifier closely reflected properties of columns, coefficients of lsers equations obtained based on in kw were employed to discuss the properties of different stationary phases. an angle and a spider diagram based on solvation energy vectors were used to compare selectivity differences between stationary phases, which provided a visual means for users to select appropriate columns with orthogonal or similar selectivity. these results of column selectivities were compared with those obtained by geometric orthogonality approach, and a consistent result was acquired. finally, click te-cd and xcharge c18pn with highest difference in column selectivity were applied to the separation of psoralea cotylifolia extraction. (c) 2014 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; solvation energy relationships ; structure-retention relationships ; supercritical-fluid chromatography ; solvatochromic comparison method ; stationary phases ; gradient elution ; molecular-mechanism ; chemometric methods ; parameter model
收录类别	SCI
语种	英语
WOS记录号	WOS:000342257200018
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145496]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jixia,Wang, Chaoran,Guo, Zhimou,et al. A novel method for characterization and comparison of reversed-phase column selectivity[J]. journal of chromatography a,2014,1361:153-161.
APA	Wang, Jixia.,Wang, Chaoran.,Guo, Zhimou.,Dong, Xuefang.,Xiao, Yuansheng.,...&Liang, Xinmiao.(2014).A novel method for characterization and comparison of reversed-phase column selectivity.journal of chromatography a,1361,153-161.
MLA	Wang, Jixia,et al."A novel method for characterization and comparison of reversed-phase column selectivity".journal of chromatography a 1361(2014):153-161.