Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection | |
Tang, Chunlan; Wang, Li; Cheng, Mengchun; Zhang, Xiaozhe; Liu, Xiaoyan; Xiao, Hongbin | |
刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences |
2014-12-15 | |
卷号 | 973页码:104-109 |
关键词 | Parishin UHPLC-FLD Metabolites Pharmacokinetics |
英文摘要 | a simple, rapid and sensitive ultra high performance liquid chromatography with fluorescence detection (uhplc-fld) method was developed and validated for quantification of parishin and its metabolites in rats. plasma samples were prepared by protein precipitation and then analyzed using uhplc-fld system. repeated optimization showed that parishin and its metabolites, including gastrodin, p-hydroxybenzyl alcohol, parishin b and parishin c, could be sensitively detected based on the autofluorescence when excitation and emission wavelengths were set at 225 nm and 295 nm, respectively. the limit of detections (lods) of gas, hba, pb, pc and pa reached 0.6, 0.8, 1, 1 and 1 ng/ml, respectively. the linearity for all targets was within the range 2.5-5000 ng/ml and the correlation coefficient (r(2)) was larger than 0.999. importantly, our method was almost free from matrix effects and the recoveries were higher than 80%. additionally, our method also had high precision and accuracy for all analytes, presenting rsds and res within +/- 6% and +/- 14%, respectively. finally, the validated uhplc-fld method was successfully applied for studying the pharmacokinetics of parishin following intragastrically administration in rats. (c) 2014 elsevier b.v. all rights reserved. |
WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
类目[WOS] | biochemical research methods ; chemistry, analytical |
研究领域[WOS] | biochemistry & molecular biology ; chemistry |
关键词[WOS] | gastrodia-elata-bl. ; pharmacokinetics ; identification ; microdialysis ; constituents ; rhizomes |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000345878800015 |
公开日期 | 2016-05-09 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/145414] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
推荐引用方式 GB/T 7714 | Tang, Chunlan,Wang, Li,Cheng, Mengchun,et al. Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2014,973:104-109. |
APA | Tang, Chunlan,Wang, Li,Cheng, Mengchun,Zhang, Xiaozhe,Liu, Xiaoyan,&Xiao, Hongbin.(2014).Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection.journal of chromatography b-analytical technologies in the biomedical and life sciences,973,104-109. |
MLA | Tang, Chunlan,et al."Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection".journal of chromatography b-analytical technologies in the biomedical and life sciences 973(2014):104-109. |
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