Characterization of an immunologically active pectin from the fruits of Lycium ruthenicum

	Characterization of an immunologically active pectin from the fruits of Lycium ruthenicum
	Peng, Qiang 1,2; Xu, Qingsong 1; Yin, Heng 1; Huang, Linjuan 3; Du, Yuguang 1
刊名	international journal of biological macromolecules
	2014-03-01
卷号	64 期号:1 页码:69-75
关键词	Lycium ruthenicum Polysaccharide Structure Pectin Immunomodulating activity
通讯作者	linjuanhuang ; 杜昱光
英文摘要	an immunologically active pectin, named lrgp5, was firstly isolated from the fruits of lycium ruthenicum murr. it contained rhamnose, arabinose, xylose, galactose and galacturonic acid in the molar ratio of 1.0:2.2:0.5:1.2:4.7. its molecular weight was estimated to be approximately 1.37 x 10(5) da by high performance gel permeation chromatography. the structure was elucidated using methylation analysis, partial acid hydrolysis, nmr and esi-ms analysis. results showed that lrgp5 consisted of a (1 -> 4)-linked galacturonic acid backbone occasionally interrupted by (1 -> 2)-linked rhamnose. the side chains were attached to position 4 of the rhamnosyl units, including (1 -> 3)-linked arabinose, (1 -> 3)-linked galactose, (1 -> 3,6)-linked galactose, (1 -> 4)-linked galacturonic acid, (1 -> 2)-linked rhamnose and (1 -> 2,4)-linked rhamnose, and the termini were arabinose and rhamnose. immunological assay results demonstrated that lrgp5 could significantly promote macrophage proliferation and enhance the secretion of nitrogen monoxide in vitro. crown copyright (c) 2013 published by elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	structural-characterization ; arabinogalactan-protein ; oligosaccharides ; polysaccharides ; l.
收录类别	SCI
语种	英语
WOS记录号	WOS:000334147300011
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144147]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycoconjugate Res Grp, Dalian 116023, Peoples R China 2.Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Peoples R China 3.NW Univ Xian, Life Sci Coll, Key Lab Resource Biol & Biotechnol Western China, Educ Minist, Xian 710069, Peoples R China
推荐引用方式 GB/T 7714	Peng, Qiang,Xu, Qingsong,Yin, Heng,et al. Characterization of an immunologically active pectin from the fruits of Lycium ruthenicum[J]. international journal of biological macromolecules,2014,64(1):69-75.
APA	Peng, Qiang,Xu, Qingsong,Yin, Heng,Huang, Linjuan,&Du, Yuguang.(2014).Characterization of an immunologically active pectin from the fruits of Lycium ruthenicum.international journal of biological macromolecules,64(1),69-75.
MLA	Peng, Qiang,et al."Characterization of an immunologically active pectin from the fruits of Lycium ruthenicum".international journal of biological macromolecules 64.1(2014):69-75.