Elevating mitochondrial reactive oxygen species by mitochondria-targeted inhibition of superoxide dismutase with a mesoporous silica nanocarrier for cancer therapy

	Elevating mitochondrial reactive oxygen species by mitochondria-targeted inhibition of superoxide dismutase with a mesoporous silica nanocarrier for cancer therapy
	Zhang, Yi 1,2; Hu, Zhengyan 1,2; Xu, Guiju 1,2; Gao, Chuanzhou 3; Wu, Ren'an 1; Zou, Hanfa 1
刊名	nano research
	2014-08-01
卷号	7 期号:8 页码:1103-1115
关键词	mitochondria reactive oxygen species apoptosis mesoporous silica nanoparticles drug delivery
通讯作者	吴仁安 ; 邹汉法
英文摘要	in the intrinsic pathway of apoptosis, stresses of mitochondrial reactive oxygen species (mitoros) might be sensed as more effective signals than those in cytosol, as mitochondria are the major sources of reactive oxygen species (ros) and pivotal components during cell apoptosis. mitochondrial superoxide dismutase (sod2) takes the leading role in eliminating mitoros, and inhibition of sod2 might induce severe disturbances overwhelming the mitochondrial oxidative equilibrium, which would elevate the intracellular oxidative stresses and drive cells to death. herein, we report a general strategy to kill cancer cells by targeted inhibition of sod2 using 2-methoxyestradiol (2-me, an inhibitor for the sod family) via a robust mitochondria-targeted mesoporous silica nanocarrier (mtmsn), with the expected elevation of mitoros and activation of apoptosis in hela cells. fe3o4@msn was employed in the mitochondria-targeted drug delivery and selective inhibition of mitochondrial enzymes, and was shown to be stable with good biocompatibility and high loading capacity. due to the selective inhibition of sod2 by 2-me/mtmsn, enhanced elevation of mitoros (132% of that with free 2-me) was obtained, coupled with higher efficiency in initiating cell apoptosis (395% of that with free 2-me in 4 h). finally, the 2-me/mtmsn exhibited powerful efficacy in targeted killing of hela cells by taking advantage of both biological recognition and magnetic guiding, causing 97.0% cell death with only 2 mu g/ml 2-me/mtmsn, hinting at its great potential in cancer therapy through manipulation of the delicate mitochondrial oxidative balance.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences ; technology
类目[WOS]	chemistry, physical ; nanoscience & nanotechnology ; materials science, multidisciplinary ; physics, applied
研究领域[WOS]	chemistry ; science & technology - other topics ; materials science ; physics
关键词[WOS]	drug-delivery ; in-vivo ; cells ; apoptosis ; ros ; nanoparticles ; therapeutics ; activation
收录类别	SCI
语种	英语
WOS记录号	WOS:000341172400002
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/143814]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Med Univ, Inst Canc Stem Cell, Dalian 116044, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yi,Hu, Zhengyan,Xu, Guiju,et al. Elevating mitochondrial reactive oxygen species by mitochondria-targeted inhibition of superoxide dismutase with a mesoporous silica nanocarrier for cancer therapy[J]. nano research,2014,7(8):1103-1115.
APA	Zhang, Yi,Hu, Zhengyan,Xu, Guiju,Gao, Chuanzhou,Wu, Ren'an,&Zou, Hanfa.(2014).Elevating mitochondrial reactive oxygen species by mitochondria-targeted inhibition of superoxide dismutase with a mesoporous silica nanocarrier for cancer therapy.nano research,7(8),1103-1115.
MLA	Zhang, Yi,et al."Elevating mitochondrial reactive oxygen species by mitochondria-targeted inhibition of superoxide dismutase with a mesoporous silica nanocarrier for cancer therapy".nano research 7.8(2014):1103-1115.