A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method

	A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method
	Huang, Junfeng 1,2; Qin, Hongqiang 1; Sun, Zhen 1,2; Huang, Guang 1,2; Mao, Jiawei 1,2; Cheng, Kai 1,2; Zhang, Zhang 1,2; Wan, Hao 1,3; Yao, Yating 1,2; Dong, Jing 1
刊名	scientific reports
	2015-05-11
卷号	5 期号:1 页码:10164
通讯作者	叶明亮 ; 邹汉法
英文摘要	enrichment of glycopeptides by hydrazide chemistry (hc) is a popular method for glycoproteomics analysis. however, possible side reactions of peptide backbones during the glycan oxidation in this method have not been comprehensively studied. here, we developed a proteomics approach to locate such side reactions and found several types of the side reactions that could seriously compromise the performance of glycoproteomics analysis. particularly, the hc method failed to identify n-terminal ser/thr glycopeptides because the oxidation of vicinal amino alcohol on these peptides generates aldehyde groups and after they are covalently coupled to hc beads, these peptides cannot be released by pngase f for identification. to overcome this drawback, we apply a peptide n-terminal protection strategy in which primary amine groups on peptides are chemically blocked via dimethyl labeling, thus the vicinal amino alcohols on peptide n-termini are eliminated. our results showed that this strategy successfully prevented the oxidation of peptide n-termini and significantly improved the coverage of glycoproteome.
学科主题	物理化学
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	hydrophilic interaction chromatography ; titanium-dioxide chromatography ; solid-phase extraction ; mass-spectrometry ; phosphoproteome analysis ; quantitative proteomics ; protein glycosylation ; linked glycoproteins ; selective enrichment ; periodate-oxidation
收录类别	SCI
语种	英语
WOS记录号	WOS:000354275000001
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/143752]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
推荐引用方式 GB/T 7714	Huang, Junfeng,Qin, Hongqiang,Sun, Zhen,et al. A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method[J]. scientific reports,2015,5(1):10164.
APA	Huang, Junfeng.,Qin, Hongqiang.,Sun, Zhen.,Huang, Guang.,Mao, Jiawei.,...&Zou, Hanfa.(2015).A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method.scientific reports,5(1),10164.
MLA	Huang, Junfeng,et al."A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method".scientific reports 5.1(2015):10164.