Gold nanoclusters-Cu2+ ensemble-based fluorescence turn-on and real-time assay for acetylcholinesterase activity and inhibitor screening

	Gold nanoclusters-Cu2+ ensemble-based fluorescence turn-on and real-time assay for acetylcholinesterase activity and inhibitor screening
	Sun,Jian; Yang,Xiurong
刊名	biosensors & bioelectronics
	2015
卷号	74 页码:177-182
关键词	SELECTIVE DETECTION CHOLINESTERASE ACTIVITY ENHANCED FLUORESCENCE METAL NANOCLUSTERS RAPID SYNTHESIS QUANTUM DOTS PROTEIN PROBE NANOPARTICLES ENZYME
通讯作者	yang,xr
英文摘要	based on the specific binding of cu2+ ions to the 11-mercaptoundecanoic acid (11-mua)-protected auncs with intense orange-red emission, we have proposed and constructed a novel fluorescent nanomaterials-metal ions ensemble at a nonfluorescence off-state. subsequently, an auncs@11-mua-cu2+ ensemble-based fluorescent chemosensor, which is amenable to convenient, sensitive, selective, turn-on and real-time assay of acetylcholinesterase (ache), could be developed by using acetylthiocholine (atch) as the substrate. herein, the sensing ensemble solution exhibits a marvelous fluorescent enhancement in the presence of ache and atch, where ache hydrolyzes its active substrate atch into thiocholine (tch), and then tch captures cu2+ from the ensemble, accompanied by the conversion from fluorescence off-state to on-state of the auncs. the ache activity could be detected less than 0.05 mu/ml within a good linear range from 0.05 to 2.5 mu/ml. our proposed fluorescence assay can be utilized to evaluate the ache activity quantitatively in real biological sample, and furthermore to screen the inhibitor of ache. as far as we know, the present study has reported the first analytical proposal for sensing ache activity in real time by using a fluorescent nanomaterials-cu2+ ensemble or focusing on the cu2+-triggered fluorescence quenching/recovery. this strategy paves a new avenue for exploring the biosensing applications of fluorescent auncs, and presents the prospect of auncs@11-mua-cu2+ ensemble as versatile enzyme activity assay platforms by means of other appropriate substrates/analytes. (c) 2015 elsevier b.v. all rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000360772800024
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64768]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Sun,Jian,Yang,Xiurong. Gold nanoclusters-Cu2+ ensemble-based fluorescence turn-on and real-time assay for acetylcholinesterase activity and inhibitor screening[J]. biosensors & bioelectronics,2015,74:177-182.
APA	Sun,Jian,&Yang,Xiurong.(2015).Gold nanoclusters-Cu2+ ensemble-based fluorescence turn-on and real-time assay for acetylcholinesterase activity and inhibitor screening.biosensors & bioelectronics,74,177-182.
MLA	Sun,Jian,et al."Gold nanoclusters-Cu2+ ensemble-based fluorescence turn-on and real-time assay for acetylcholinesterase activity and inhibitor screening".biosensors & bioelectronics 74(2015):177-182.