Poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for specific recognition and release of lysozyme

	Poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for specific recognition and release of lysozyme
	Ji, Shilei 1,4; Li, Nan 1,2; Shen, Yong 2,3; Li, Qi 1; Qiao, Juan 1; Li, Zhibo 3
刊名	ANALYTICA CHIMICA ACTA
	2016-02-16
卷号	909 页码:60-66
关键词	Poly(amino acid) Thermoresponsive Molecularly imprinted polymers (MIPs) Surface imprinting magnetic particles Lysozyme
英文摘要	In this study, poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for recognition and release of lysozyme was prepared via surface imprinting method. For constructing the molecularly imprinted polymer (MIP) layer, amino acid-based thermoresponsive monomer (N-methacryloyl-L-alanine methyl ester, MA-L-Ala-OMe) was mainly selected for the functional monomer along with N, N'-methylenebis(acrylamide) as the crosslinker. The resultant magnetic MIP nanoparticles were characterized in detail. Meanwhile, the dynamic light scattering studies and swelling ratios measurements were carried out for demonstrating the thermoresponsive property of the imprinted nanoparticles. The prepared magnetic MIP nanoparticles showed good adsorption capacity and selective recognition properties to lysozyme. Moreover, the fast adsorption process could reach equilibrium within 15 min. Importantly, the capture and release of lysozyme could be easily realized simply by altering the temperature of aqueous solution. Furthermore, the prepared imprinted nanoparticles were applied to separate lysozyme from the real egg white samples. The results proved that the thermoresponsive MIPs based on MA-L-Ala-OMe have great potential for selectively enriching target proteins in real samples. (C) 2016 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/30074]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Chem, Lab Polymer Phys & Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 4.Shandong Agr Univ, Grad Sch, Jinan 271018, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Ji, Shilei,Li, Nan,Shen, Yong,et al. Poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for specific recognition and release of lysozyme[J]. ANALYTICA CHIMICA ACTA,2016,909:60-66.
APA	Ji, Shilei,Li, Nan,Shen, Yong,Li, Qi,Qiao, Juan,&Li, Zhibo.(2016).Poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for specific recognition and release of lysozyme.ANALYTICA CHIMICA ACTA,909,60-66.
MLA	Ji, Shilei,et al."Poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for specific recognition and release of lysozyme".ANALYTICA CHIMICA ACTA 909(2016):60-66.