Dissolution/diffusion-based Injection for Fast Separation of Epinephrine and Norepinephrine by Short Capillary Electrophoresis

	Dissolution/diffusion-based Injection for Fast Separation of Epinephrine and Norepinephrine by Short Capillary Electrophoresis
	Hu Can 1; Chen Yi 1,2
刊名	CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
	2015-09-10
卷号	36 期号:9 页码:1681-1686
关键词	Micrometer-zonal injection Fast short capillary electrophoresis Epinephrine Amino acid Separation
英文摘要	Shortening capillary is an effective strategy to speed up capillary electrophoresis(CE), but a very fine injection approach must be availableto obtain an ultra-short initial zone which is remains a big challenge. To conquer it, an easy injection approach called dissolution/diffusion-based injection(DDI) was explored simply based on the properties of an analyte able to dissolve and diffuse into a solution. DDI was easily operated by letting the capillary inlet touch either a sample solution or liquid-dried film, giving initial sample zones <15 mu m. The resulted DDI-CE is reproducible, with relative standard deviation of peak time below 1. 6%, and was shown to be applicable to the fast (40 s) separation of epinephrine and norepinephrine mixtures through only 3 cm effective length under 400 V/cm. Its applicability was further validated by quantification of epinephrine injection.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28825]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Hu Can,Chen Yi. Dissolution/diffusion-based Injection for Fast Separation of Epinephrine and Norepinephrine by Short Capillary Electrophoresis[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2015,36(9):1681-1686.
APA	Hu Can,&Chen Yi.(2015).Dissolution/diffusion-based Injection for Fast Separation of Epinephrine and Norepinephrine by Short Capillary Electrophoresis.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,36(9),1681-1686.
MLA	Hu Can,et al."Dissolution/diffusion-based Injection for Fast Separation of Epinephrine and Norepinephrine by Short Capillary Electrophoresis".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 36.9(2015):1681-1686.