A multi-enzyme microreactor-based online electrochemical system for selective and continuous monitoring of acetylcholine

	A multi-enzyme microreactor-based online electrochemical system for selective and continuous monitoring of acetylcholine
	Lin, Yuqing; Yu, Ping; Mao, Lanqun
刊名	ANALYST
	2015
卷号	140 期号:11 页码:3781-3787
英文摘要	This study demonstrates an online electrochemical system (OECS) for selective and continuous measurements of acetylcholine (ACh) through efficiently integrating in vivo microdialysis, a multi-enzyme micro-reactor and an electrochemical detector. A multi-enzyme microreactor was prepared first by co-immobilizing two kinds of enzymes, i.e. choline oxidase (ChOx) and catalase (Cat), onto magnetite nanoparticles and then confining the as-formed nanoparticles into a fused-silica capillary with the assistance of an external magnet. The multi-enzyme microreactor was settled between an in vivo microdialysis sampling system and an electrochemical detector to suppress the interference from choline toward ACh detection. Selective detection of ACh was accomplished using the electrochemical detector with ACh esterase (AChE) and ChOx as the recognition units for ACh and Prussian blue (PB) as the electrocatalyst for the reduction of hydrogen peroxide (H2O2). The current recorded with the OECS was linear with the concentration of ACh (I/nA = -3.90C(ACh)/mu M + 1.21, gamma = 0.998) within a concentration range of 5 mu M to 100 mu M. The detection limit, based on a signal-to-noise ratio of 3, was calculated to be 1 mu M. Interference investigation demonstrates that the OECS did not produce an observable current response toward physiological levels of common electroactive species, such as ascorbic acid (AA), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), and uric acid (UA). The high selectivity and the good linearity in combination with the high stability may enable the OECS developed here as a potential system for continuous monitoring of cerebral ACh release in some physiological and pathological processes.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28095]
专题	化学研究所_活体分析化学实验室
作者单位	Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Lin, Yuqing,Yu, Ping,Mao, Lanqun. A multi-enzyme microreactor-based online electrochemical system for selective and continuous monitoring of acetylcholine[J]. ANALYST,2015,140(11):3781-3787.
APA	Lin, Yuqing,Yu, Ping,&Mao, Lanqun.(2015).A multi-enzyme microreactor-based online electrochemical system for selective and continuous monitoring of acetylcholine.ANALYST,140(11),3781-3787.
MLA	Lin, Yuqing,et al."A multi-enzyme microreactor-based online electrochemical system for selective and continuous monitoring of acetylcholine".ANALYST 140.11(2015):3781-3787.