In vivo and continuous measurement of bisulfide in the hippocampus of rat's brain by an on-line integrated microdialysis/droplet-based microfluidic system | |
Gu, Feidan1; Zhou, Xiaoyu1; Zhu, Xiaocui1; Zhao, Meiping1; Hao, Jie2; Yu, Ping2; Mao, Lanqun2 | |
刊名 | ANALYST |
2015 | |
卷号 | 140期号:11页码:3814-3819 |
英文摘要 | An on-line and continuous approach was demonstrated for in vivo measurement of bisulfide in rat's brain. A modified droplet-based microfluidic system was constructed, which allowed on-line qualification of the fluorescence responses of the gold nanoparticle-glutathione-fluorescein isothiocyanate probe to the variation of bisulfide in the presence of the cerebral microdialysate background. The on-line method achieved a dynamic working range from 5.0 mu M to 40 mu M and a detection limit of 2.5 mu M. The in vivo bisulfide concentration in the hippocampus of rat's brain was measured under different physiological conditions. The on-line method may facilitate the study of H2S biology by providing a previously unattainable continuous record of H2S variation in living animals. It also provides a practical platform for in vivo and continuous monitoring of other important species in cerebral systems. |
收录类别 | SCI |
语种 | 英语 |
公开日期 | 2016-05-09 |
内容类型 | 期刊论文 |
源URL | [http://ir.iccas.ac.cn/handle/121111/28065] |
专题 | 化学研究所_活体分析化学实验室 |
作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Gu, Feidan,Zhou, Xiaoyu,Zhu, Xiaocui,et al. In vivo and continuous measurement of bisulfide in the hippocampus of rat's brain by an on-line integrated microdialysis/droplet-based microfluidic system[J]. ANALYST,2015,140(11):3814-3819. |
APA | Gu, Feidan.,Zhou, Xiaoyu.,Zhu, Xiaocui.,Zhao, Meiping.,Hao, Jie.,...&Mao, Lanqun.(2015).In vivo and continuous measurement of bisulfide in the hippocampus of rat's brain by an on-line integrated microdialysis/droplet-based microfluidic system.ANALYST,140(11),3814-3819. |
MLA | Gu, Feidan,et al."In vivo and continuous measurement of bisulfide in the hippocampus of rat's brain by an on-line integrated microdialysis/droplet-based microfluidic system".ANALYST 140.11(2015):3814-3819. |
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