Recognition of extended linear and cyclised polyketide mimics by a type II acyl carrier protein

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	Recognition of extended linear and cyclised polyketide mimics by a type II acyl carrier protein
	Dong, Xu 1,2; Bailey, Christopher D.1,3; Williams, Christopher 1; Crosby, John 1; Simpson, Thomas J.1; Willis, Christine L.1; Crump, Matthew P.1
刊名	CHEMICAL SCIENCE
	2016
卷号	7 期号:3 页码:1779-1785
英文摘要	Polyketides are secondary metabolites which display both valuable pharmaceutical and agrochemical properties. Biosynthesis is performed by polyketide synthases (PKSs), and the acyl carrier protein (ACP), a small acidic protein, that transports the growing polyketide chain and is essential for activity. Here we report the synthesis of two aromatic probes and a linear octaketide mimic that have been tethered to actinorhodin ACP. These experiments were aimed at probing the ACP's capacity to sequester a non-polar versus a phenolic aromatic ring (that more closely mimics a polyketide intermediate) as well as investigations with extended polyketide chain surrogates. The binding of these mimics has been assessed using high-resolution solution NMR studies and high-resolution structure determination. These results reveal that surprisingly a PKS ACP is able to bind and sequester a bulky non-polar substrate containing an aromatic ring in a fatty acid type binding mode, but the introduction of even a small degree of polarity favours a markedly different association at a surface site that is distinct from that employed by fatty acid ACPs.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	STREPTOMYCES-COELICOLOR ; ACTINORHODIN ; SYNTHASE ; BIOSYNTHESIS ; ACP ; CLONING ; REVEAL ; LENGTH ; A3(2)
收录类别	SCI
语种	英语
WOS记录号	WOS:000371021900015
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/9218]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Univ Bristol, Sch Chem, Cantocks Close, Bristol BS8 1TS, Avon, England 2.Chinese Acad Sci, Wuhan Inst Phys & Math, CAS Key Lab Magnet Resonance Biol Syst, Wuhan 430071, Hubei Province, Peoples R China 3.Fyfield Business & Res Pk, Fyfield Rd, Ongar CM5 0GS, Essex, England
推荐引用方式 GB/T 7714	Dong, Xu,Bailey, Christopher D.,Williams, Christopher,et al. Recognition of extended linear and cyclised polyketide mimics by a type II acyl carrier protein[J]. CHEMICAL SCIENCE,2016,7(3):1779-1785.
APA	Dong, Xu.,Bailey, Christopher D..,Williams, Christopher.,Crosby, John.,Simpson, Thomas J..,...&Crump, Matthew P..(2016).Recognition of extended linear and cyclised polyketide mimics by a type II acyl carrier protein.CHEMICAL SCIENCE,7(3),1779-1785.
MLA	Dong, Xu,et al."Recognition of extended linear and cyclised polyketide mimics by a type II acyl carrier protein".CHEMICAL SCIENCE 7.3(2016):1779-1785.