A Homogeneous Signal-On Strategy for the Detection of rpoB Genes of Mycobacterium tuberculosis Based on Electrochemiluminescent Graphene Oxide and Ferrocene Quenching

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 离职团队

	A Homogeneous Signal-On Strategy for the Detection of rpoB Genes of Mycobacterium tuberculosis Based on Electrochemiluminescent Graphene Oxide and Ferrocene Quenching
	Li, F (Li, Fang) ; Yu, YQ (Yu, Yuqi) ; Li, Q (Li, Qi) ; Zhou, M (周明) ; Cui, H (Cui, Hua)
刊名	Cui, H (Cui, Hua)
	2014
卷号	86 期号:3 页码:1608-1613
关键词	MUTATIONS PLATFORM PCR
通讯作者	Cui, H (Cui, Hua)
英文摘要	Tuberculosis (TB) remains one of the leading causes of morbidity and mortality all over the world and multidrug resistance TB (MDR-TB) pose a serious threat to the TB control and represent an increasing public health problem. In this work, we report a homogeneous signal-on electrochemiluminescence (ECL) DNA sensor for the sensitive and specific detection of rpoB genes of MDR-TB by using ruthenium(II) complex functionalized graphene oxide (Ru-GO) as suspension sensing interface and ferrocene-labeled ssDNA (Fc-ssDNA) as ECL intensity controller. The ECL of Ru-GO could be effectively quenched by Fc-ssDNA absorbed on the Ru-GO nanosheets. The Ru-GO has good discrimination ability over ssDNA and dsDNA. Mutant ssDNA target responsible for the drug resistant tuberculosis can hybridize with Fc-ssDNA and release Fc-ssDNA from Ru-GO surface, leading to the recovery of ECL. Mutant ssDNA target can be detected in a range from 0.1 to 100 nM with a detection limit of 0.04 nM. The proposed protocol is sensitive, specific, simple, time-saving and polymerase chain reaction free without complicated immobilization, separation and washing steps, which creates a simple but valuable tool for facilitating fast and accurate detection of disease related specific sequences or gene mutations.
收录类别	SCI
语种	英语
WOS记录号	WOS:000331014800042
公开日期	2015-02-03
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1770]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_离职团队
推荐引用方式 GB/T 7714	Li, F ,Yu, YQ ,Li, Q ,et al. A Homogeneous Signal-On Strategy for the Detection of rpoB Genes of Mycobacterium tuberculosis Based on Electrochemiluminescent Graphene Oxide and Ferrocene Quenching[J]. Cui, H (Cui, Hua),2014,86(3):1608-1613.
APA	Li, F ,Yu, YQ ,Li, Q ,Zhou, M ,&Cui, H .(2014).A Homogeneous Signal-On Strategy for the Detection of rpoB Genes of Mycobacterium tuberculosis Based on Electrochemiluminescent Graphene Oxide and Ferrocene Quenching.Cui, H (Cui, Hua),86(3),1608-1613.
MLA	Li, F ,et al."A Homogeneous Signal-On Strategy for the Detection of rpoB Genes of Mycobacterium tuberculosis Based on Electrochemiluminescent Graphene Oxide and Ferrocene Quenching".Cui, H (Cui, Hua) 86.3(2014):1608-1613.