Accelerated Postero-Lateral Spinal Fusion by Collagen Scaffolds Modified with Engineered Collagen-Binding Human Bone Morphogenetic Protein-2 in Rats

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 戴建武团队

	Accelerated Postero-Lateral Spinal Fusion by Collagen Scaffolds Modified with Engineered Collagen-Binding Human Bone Morphogenetic Protein-2 in Rats
	Han, XL; Zhang, W; Gu, J; Zhao, H; Ni, L; Han, JJ; Zhou, Y; Gu, YN; Zhu, XS; Sun, J
刊名	PLOS ONE
	2014-05-28
卷号	9 期号:5
关键词	LONG-TERM DELIVERY RABBIT MODEL RHBMP-2 MATRIX REGENERATION BMP-2 CARRIER GRAFT OSTEOGENESIS ARTHRODESIS
通讯作者	戴建武
英文摘要	Bone morphogenetic protein-2 (BMP-2) is a potent osteoinductive cytokine that plays a critical role in bone regeneration and repair. However, its distribution and side effects are major barriers to its success as therapeutic treatment. The improvement of therapy using collagen delivery matrices has been reported. To investigate a delivery system on posterolateral spinal fusion, both engineered human BMP-2 with a collagen binding domain (CBD-BMP-2) and collagen scaffolds were developed and their combination was implanted into Sprague-Dawley (SD) rats to study Lumbar 4-5 (L4-L5) posterolateral spine fusion. We divided SD rats into three groups, the sham group (G1, n = 20), the collagen scaffold-treated group (G2, n = 20) and the BMP-2-loaded collagen scaffolds group (G3, n = 20). 16 weeks after surgery, the spines of the rats were evaluated by X-radiographs, high-resolution micro-computed tomography (micro-CT), manual palpation and hematoxylin and eosin (H&E) staining. The results showed that spine L4-L5 fusions occurred in G2(40%) and G3(100%) group, while results from the sham group were inconsistent. Moreover, G3 had better results than G2, including higher fusion efficiency (X score, G2 = 2.4 +/- 0.163, G3 = 3.0 +/- 0, p<0.05), higher bone mineral density (BMD, G2: 0.3337 +/- 0.0025g/cm3, G3: 0.4353 +/- 0.0234g/cm3, p<0.05) and more bone trabecular formation. The results demonstrated that with site-specific collagen binding domain, a dose of BMP-2 as low as 0.02mg CBD-BMP-2/cm(3) collagen scaffold could enhance the posterolateral intertransverse process fusion in rats. It suggested that combination delivery could be an alternative in spine fusion with dramatically decreased side effects caused by high dose of BMP-2.
收录类别	SCI
语种	英语
WOS记录号	WOS:000336858300047
公开日期	2014-12-08
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1671]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队
推荐引用方式 GB/T 7714	Han, XL,Zhang, W,Gu, J,et al. Accelerated Postero-Lateral Spinal Fusion by Collagen Scaffolds Modified with Engineered Collagen-Binding Human Bone Morphogenetic Protein-2 in Rats[J]. PLOS ONE,2014,9(5).
APA	Han, XL.,Zhang, W.,Gu, J.,Zhao, H.,Ni, L.,...&Shi, Q.(2014).Accelerated Postero-Lateral Spinal Fusion by Collagen Scaffolds Modified with Engineered Collagen-Binding Human Bone Morphogenetic Protein-2 in Rats.PLOS ONE,9(5).
MLA	Han, XL,et al."Accelerated Postero-Lateral Spinal Fusion by Collagen Scaffolds Modified with Engineered Collagen-Binding Human Bone Morphogenetic Protein-2 in Rats".PLOS ONE 9.5(2014).