Rapid and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome Virus on Nanoscale by Biosensor Based on Imaging Ellipsometry

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 戴建武团队

	Rapid and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome Virus on Nanoscale by Biosensor Based on Imaging Ellipsometry
	Chen, YY(陈艳艳)
刊名	INTEGRATED FERROELECTRICS
	2013
卷号	145 期号:1 页码:122-129
通讯作者	Huo, DQ
英文摘要	This study describes a simple and rapid method for virus detection on nanoscale measurement utilizing biosensor based on imaging ellipsometry. Porcine reproductive and respiratory syndrome virus (PRRSV) was served as an experimental model for demonstration. Staphylococcal protein A was firstly attached onto carboxyl-modified silicon substrate and purified anti-PRRSV IgG obtained from hyperimmune sera combined with Protein A for oriented immobilization to form capture layer. PRRSV in samples can then be specifically grabbed onto the capture layer. The signal (grayscale value) after virus combination is intuitively quantified by imaging ellipsometry. The minimum detection limit was as low as 2.4 x 10(3) median cell infectious dose (CCID50 mL(-1)) infectious virus. Application the proposed method to 20 clinic samples demonstrated that the result was significant correlated (R = 0.85) with virus isolation. The detection time was only 20min expended.
收录类别	SCI
语种	英语
WOS记录号	WOS:000321687200016
公开日期	2014-01-20
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1534]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队
推荐引用方式 GB/T 7714	Chen, YY. Rapid and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome Virus on Nanoscale by Biosensor Based on Imaging Ellipsometry[J]. INTEGRATED FERROELECTRICS,2013,145(1):122-129.
APA	Chen, YY.(2013).Rapid and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome Virus on Nanoscale by Biosensor Based on Imaging Ellipsometry.INTEGRATED FERROELECTRICS,145(1),122-129.
MLA	Chen, YY."Rapid and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome Virus on Nanoscale by Biosensor Based on Imaging Ellipsometry".INTEGRATED FERROELECTRICS 145.1(2013):122-129.