Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 戴建武团队

	Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair
	Dai, JW(戴建武)
刊名	BIOMATERIALS
	2013-07
卷号	34 期号:21 页码:5107-5116
关键词	Collagen scaffold EGFR antibody functionalization Neural progenitor cells Neuronal differentiation Myelin proteins Spinal cord injury repair
通讯作者	Dai, JW(戴建武)
英文摘要	The main challenge for neural progenitor cell (NPC)-mediated repair of spinal cord injury (SCI) is lack of favorable environment to direct its differentiation towards neurons rather than glial cells. The myelin associated inhibitors have been demonstrated to promote NPC differentiation into glial lineage. Herein, to inhibit the downstream signaling activated by myelin associated inhibitors, cetuximab, an epidermal growth factor receptor (EGFR) neutralizing antibody, functionalized collagen scaffold has been developed as a vehicle for NPC implantation. It was found that collagen-cetuximab 1 mu g scaffolds enhanced neuronal differentiation and inhibited astrocytic differentiation of NPCs exposed to myelin proteins significantly in vitro. To test the therapeutic effect in vivo, NPCs expressing green fluorescent protein (GFP)-embedded scaffolds have been implanted into the 4 mm-long hemisection lesion of rats. We found that the collagen-cetuximab 5 mu g scaffolds induced neuronal differentiation and decreased astrocytic differentiation of NPCs, enhanced axon regeneration, and promoted functional recovery markedly. A well-functionalized scaffold was constructed to improve the recovery of SCI, which could promote the neuronal differentiation of neural progenitor cells in vivo. (C) 2013 Elsevier Ltd. All rights reserved.
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000319630000020
公开日期	2014-01-20
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1529]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队
通讯作者	Dai, JW(戴建武)
推荐引用方式 GB/T 7714	Dai, JW. Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair[J]. BIOMATERIALS,2013,34(21):5107-5116.
APA	Dai, JW.(2013).Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair.BIOMATERIALS,34(21),5107-5116.
MLA	Dai, JW."Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair".BIOMATERIALS 34.21(2013):5107-5116.