The osteogenic effect of bone morphogenetic protein-2 on the collagen scaffold conjugated with antibodies

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 戴建武团队

	The osteogenic effect of bone morphogenetic protein-2 on the collagen scaffold conjugated with antibodies
	Wang QB (王强斌); Dai JW (戴建武)
刊名	Journal of Controlled Release
	2010-01-04
卷号	141 期号:1 页码:30-37
关键词	Collagen Bone morphogenetic protein Antibody Bone regeneration
通讯作者	Dai JW (戴建武)
合作状况	其它
英文摘要	Considerable research has been focused on the exploration of bone morphogenetic protein-2 (BMP(2)) delivery vehicles for achieving prolonged availability and maintaining efficient local concentration at the bone injury sites. In this study, heterobifunctional cross-linkers Sulfo-SMCC and cyclic thioimidate compound Traut's Reagent were used to conjugate monoclonal polyhistidine antibody on collagen scaffold demineralized bone matrix (DBM) to create specific binding between BMP(2) containing six histidines tag (His-BMP(2)) and DBM. According to the optimized cross-linking reagent concentration, more polyhistidine antibodies conjugated on DBM with 5 mg/ml Traut's Reagent and 25 ug/ml Sulfo-SMCC than physical adsorption. Monoclonal antibodies conjugated DBM (MAbs-DBM) could bind more His-BMP(2) than DBM and achieved controlled release in vitro. The alkaline phophatase (AP) activity of C2C12 cells on MAbs-DBM indicated that His-BMP(2) retained on MAbs-DBM preserved the function to induce osteogenic differentiation. His-BMPZ/MAbs-DBM induced more ectopic bone formation (AP activity assay and histochemistry stain) than control group after subcutaneous implantation. The results demonstrated that antibody-collagen system could be useful for maintaining higher local therapy concentration of growth factors at the injury sites.
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000274104200005
公开日期	2011-03-11
内容类型	期刊论文
源URL	[http://58.210.77.100/handle/332007/332]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_王强斌团队
通讯作者	Dai JW (戴建武)
推荐引用方式 GB/T 7714	Wang QB ,Dai JW . The osteogenic effect of bone morphogenetic protein-2 on the collagen scaffold conjugated with antibodies[J]. Journal of Controlled Release,2010,141(1):30-37.
APA	Wang QB ,&Dai JW .(2010).The osteogenic effect of bone morphogenetic protein-2 on the collagen scaffold conjugated with antibodies.Journal of Controlled Release,141(1),30-37.
MLA	Wang QB ,et al."The osteogenic effect of bone morphogenetic protein-2 on the collagen scaffold conjugated with antibodies".Journal of Controlled Release 141.1(2010):30-37.