Immunogold labeling and X-ray fluorescence microscopy reveal enrichment ratios of Cu and Zn, metabolism of APP and amyloid-beta plaque formation in a mouse model of Alzheimer's disease

	Immunogold labeling and X-ray fluorescence microscopy reveal enrichment ratios of Cu and Zn, metabolism of APP and amyloid-beta plaque formation in a mouse model of Alzheimer's disease
	Wang HJ(王华建); Wang M(王萌); Wang B(汪冰); Wang, HJ; Wang, M; Wang, B; Li, M; Chen, HQ; Yu, XH; Yang, K
刊名	METALLOMICS
	2012
卷号	4 期号:10 页码:1113-1118
英文摘要	The mechanism that triggers amyloid-beta (A beta) fibrillation and aggregation is still elusive. Evidence suggests that the extensional interactions of the amyloid precursor protein (APP) and A beta with transition biometals, copper (Cu) and zinc (Zn), may be key occurrences in the processes of A beta aggregation and toxicity. By using an immunogold labeling technique combined with synchrotron radiation X-ray fluorescence microprobe (SR-mu XRF) scanning analysis, the profiles of APP, A beta 42 and Cu, Zn in the brain of APP transgenic mouse with the development of the disease were characterized. This investigation provides visual, kinetic and spatial evidence of the correlation of APP and A beta-metals in AD brain sections. The visual evidence demonstrates the association of metals Cu and Zn with A beta 42 during plaque formation, which helps implicate the role of metal ion homeostasis in human AD pathology.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
WOS记录号	WOS:000310123100012
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/224106]
专题	高能物理研究所_院士高能物理研究所_粒子天体物理中心高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Wang HJ,Wang M,Wang B,et al. Immunogold labeling and X-ray fluorescence microscopy reveal enrichment ratios of Cu and Zn, metabolism of APP and amyloid-beta plaque formation in a mouse model of Alzheimer's disease[J]. METALLOMICS,2012,4(10):1113-1118.
APA	王华建.,王萌.,汪冰.,Wang, HJ.,Wang, M.,...&丰伟悦.(2012).Immunogold labeling and X-ray fluorescence microscopy reveal enrichment ratios of Cu and Zn, metabolism of APP and amyloid-beta plaque formation in a mouse model of Alzheimer's disease.METALLOMICS,4(10),1113-1118.
MLA	王华建,et al."Immunogold labeling and X-ray fluorescence microscopy reveal enrichment ratios of Cu and Zn, metabolism of APP and amyloid-beta plaque formation in a mouse model of Alzheimer's disease".METALLOMICS 4.10(2012):1113-1118.