Copper Uptake and Its Effect on Metal Distribution in Root Growth Zones of Commelina communis Revealed by SRXRF

	Copper Uptake and Its Effect on Metal Distribution in Root Growth Zones of Commelina communis Revealed by SRXRF
	Shi, JY; Yuan, XF; Chen, XC; Wu, B; Huang, YY; Chen, YX; Huang YY(黄宇营)
刊名	BIOLOGICAL TRACE ELEMENT RESEARCH
	2011
卷号	141 期号:1-3 页码:#REF!
关键词	Commelina communis Copper uptake Distribution Root growth zones SRXRF
通讯作者	Shi, JY (reprint author), Zhejiang Univ, Dept Environm Engn, Huajiachi Campus,268 Kaixuan Rd, Hangzhou 310029, Zhejiang, Peoples R China.
英文摘要	To explore the copper uptake mechanisms by the Cu-tolerant plant Commelina communis, the contents of Cu and other metals (including Fe, Zn, and Mn) in roots were detected using atomic absorption spectrometer under transporter inhibitors, partial element deficiency, or Cu excess treatments, while distribution characters of Cu and other metals in root growth zones were investigated by synchrotron radiation X-ray fluorescence spectroscopy (SRXRF). Cu uptake was inhibited by the uncoupler DNP and P-type ATPase inhibitor Na(3)VO(4), not by the Ca(2+) ion channel inhibitor LaCl(3), suggesting that Cu could probably be assimilated actively by root and be related with P-type ATPase, but not through Ca(2+) ion channel. Fe or Zn deficiency could enhance Cu uptake, while 100 mu M Cu inhibited Fe, Zn, and Mn accumulation in roots significantly. Metal distribution under 100 mu M Cu treatment was investigated by SRXRF. High level of Cu was found in the root meristem, and higher Cu concentrations were observed in the vascular cylinder than those in the endodermis, further demonstrating the initiative Cu transport in the root of C. communis. Under excess Cu stress, most Fe was located in the epidermis, and Fe concentrations in the endodermis were higher than those in the vascular cylinder, suggesting Cu and Fe competition not only in the epidermal cells but also for the intercellular and intracellular transport in roots. Zn was present in the meristem and the vascular cylinder similar to Cu. Cu and Zn showed a similar pattern. Mn behaves as Zn does, but not like Fe.
学科主题	Biochemistry & Molecular Biology; Endocrinology & Metabolism
类目[WOS]	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
收录类别	SCI
WOS记录号	WOS:000290727800031
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/227472]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Shi, JY,Yuan, XF,Chen, XC,et al. Copper Uptake and Its Effect on Metal Distribution in Root Growth Zones of Commelina communis Revealed by SRXRF[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2011,141(1-3):#REF!.
APA	Shi, JY.,Yuan, XF.,Chen, XC.,Wu, B.,Huang, YY.,...&黄宇营.(2011).Copper Uptake and Its Effect on Metal Distribution in Root Growth Zones of Commelina communis Revealed by SRXRF.BIOLOGICAL TRACE ELEMENT RESEARCH,141(1-3),#REF!.
MLA	Shi, JY,et al."Copper Uptake and Its Effect on Metal Distribution in Root Growth Zones of Commelina communis Revealed by SRXRF".BIOLOGICAL TRACE ELEMENT RESEARCH 141.1-3(2011):#REF!.