An X-ray absorption spectroscopy investigation of speciation and biotransformation of copper in Elsholtzia splendens

	An X-ray absorption spectroscopy investigation of speciation and biotransformation of copper in Elsholtzia splendens
	Shi, JY; Wu, B; Yuan, XF; Cao, YY; Chen, XC; Chen, YX; Hu, TD; Hu TD(胡天斗)
刊名	PLANT AND SOIL
	2008
卷号	302 期号:1-2 页码:#REF!
关键词	Elsholtzia splendens copper cell wall ligands X-ray absorption spectroscopy
通讯作者	Shi, JY (reprint author), Zhejiang Univ, Dept Environm Engn, Huajiachi Campus,268 Kaixuan Rd, Hangzhou 310029, Zhejiang, Peoples R China.
英文摘要	Elsholtzia splendens is a Cu-tolerant plant growing in copper mine areas in the south of China. In this study, X-ray absorption spectroscopy (XAS) was used to investigate the Cu speciation and biotransformation in E. splendens with 300 mu M Cu treatment from 10 days to 60 days. The results showed that 300 mu M Cu was phytotoxic to E. spendens. The Cu K-edge X-ray absorption near edge structure (XANES) revealed that most copper in roots, stems and leaves exists as divalent Cu. Cu speciation changed depending on the treatment time, but there was no unidirectional trend in roots, stems, and leaves. The percentages of potential Cu ligands in all samples were estimated by fitting the XANES spectra with linear combinations. Most Cu in roots, stems and leaves was bound with cell wall and histidine (His)-like ligands, while a minor proportion of the Cu was bound to oxalate and glutathione-like ligands. The fitting results of Cu K-edge extended X-ray absorption fine structure (EAXFS) showed that nitrogen/oxygen (N/O) ligands were dominant in roots, stems and leaves of the plant, while S ligands were rare. All these results suggest that Cu bound by N/O ligands plays a key role in Cu detoxification of E. splendens, and a role for classical metal-detoxifying S ligands, such as metallothioneins and phytochelatins, in Cu detoxification of E. splendens is not supported in the present study. Due to the phytotoxicity of 300 mu M Cu to E. splendens, the question of whether S ligands play a significant role in Cu detoxification in E. splendens exposed to lower levels of Cu should be further studied.
学科主题	Agriculture; Plant Sciences
类目[WOS]	Agronomy ; Plant Sciences ; Soil Science
收录类别	SCI
WOS记录号	WOS:000251971500013
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/226611]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Shi, JY,Wu, B,Yuan, XF,et al. An X-ray absorption spectroscopy investigation of speciation and biotransformation of copper in Elsholtzia splendens[J]. PLANT AND SOIL,2008,302(1-2):#REF!.
APA	Shi, JY.,Wu, B.,Yuan, XF.,Cao, YY.,Chen, XC.,...&胡天斗.(2008).An X-ray absorption spectroscopy investigation of speciation and biotransformation of copper in Elsholtzia splendens.PLANT AND SOIL,302(1-2),#REF!.
MLA	Shi, JY,et al."An X-ray absorption spectroscopy investigation of speciation and biotransformation of copper in Elsholtzia splendens".PLANT AND SOIL 302.1-2(2008):#REF!.