Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase

	Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase
	Liang, YJ; Gao ZQ(高增强); Dong YH(董宇辉); 刘全胜;Gao, ZQ; Dong, YH; Liu, QS
刊名	PROTEIN SCIENCE
	2014
卷号	23 期号:10 页码:1442-1450
关键词	alkylsulfatase hydrolase metalloenzyme X-ray crystallography metallo-beta-lactamase
英文摘要	Sodium dodecyl sulfate (SDS) is a widely used anionic surfactant in industry and research settings, and is known to have a detrimental effect to the environment. The pathway of SDS degradation by bacteria is initiated by an alkylsulfatase and the oxidized product, 1-dodecanoic acid, subsequently enters into the beta-oxidation pathway and is used as a carbon source. In this work, we solved the crystal structure of Escherichia coli uncharacterized protein YjcS and identified that it belongs to the Type III alkylsulfatase with a signal peptide (residues 1-29) at the N terminus. YjcS hydrolyzed SDS and the double mutant D184N-H185A located in the conserved HXHXDH catalytic motif abolished this activity.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
WOS记录号	WOS:000342210600012
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/225317]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Liang, YJ,Gao ZQ,Dong YH,et al. Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase[J]. PROTEIN SCIENCE,2014,23(10):1442-1450.
APA	Liang, YJ,高增强,董宇辉,刘全胜；Gao, ZQ,Dong, YH,&Liu, QS.(2014).Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase.PROTEIN SCIENCE,23(10),1442-1450.
MLA	Liang, YJ,et al."Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase".PROTEIN SCIENCE 23.10(2014):1442-1450.