High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S-cerevisiae RNA endonuclease Swt1

	High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S-cerevisiae RNA endonuclease Swt1
	彭淑霞;Peng, SX; Zhou, K; Wang, WJ; Gao, ZQ; Dong, YH; Liu, QS
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2014
卷号	453 期号:4 页码:826-832
关键词	HEPN domain Crystal structure RNA endonuclease SAXS Swt1
英文摘要	Swt1 is an RNA endonuclease that plays an important role in quality control of nuclear messenger ribonucleoprotein particles (mRNPs) in eukaryotes; however, its structural details remain to be elucidated. Here, we report the crystal structure of the C-terminal (CT) domain of Swt1 from Saccharomyces cerevisiae, which shares common characteristics of higher eukaryotes and prokaryotes nucleotide binding (HEPN) domain superfamily. To study in detail the full-length protein structure, we analyzed the low-resolution architecture of Swt1 in solution using small angle X-ray scattering (SAXS) method. Both the CT domain and middle domain exhibited a good fit upon superimposing onto the molecular envelope of Swt1. Our study provides the necessary structural information for detailed analysis of the functional role of Swt1, and its importance in the process of nuclear mRNP surveillance. (C) 2014 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
收录类别	SCI
WOS记录号	WOS:000346329000025
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/224857]
专题	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	彭淑霞；Peng, SX,Zhou, K,Wang, WJ,et al. High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S-cerevisiae RNA endonuclease Swt1[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,453(4):826-832.
APA	彭淑霞；Peng, SX,Zhou, K,Wang, WJ,Gao, ZQ,Dong, YH,&Liu, QS.(2014).High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S-cerevisiae RNA endonuclease Swt1.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,453(4),826-832.
MLA	彭淑霞；Peng, SX,et al."High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S-cerevisiae RNA endonuclease Swt1".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 453.4(2014):826-832.