Separation and purification of fullerenols for improved biocompatibility | |
Li J(李娟); Zhang MY(张铭倚); Sun BY(孙宝云); Li, J; Zhang, MY; Sun, BY; Xing, GM; Song, Y; Guo, HL; Chang, YA | |
刊名 | CARBON |
2012 | |
卷号 | 50期号:2页码:460-469 |
英文摘要 | Isoelectric focusing was used to separate the different components of fullerenols based on their different electronegativities induced by the surface chemical functionalities and structures. Purified fractions of fullerenols were analyzed and characterized by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The results showed that four fractions of fullerenols could be separated by isoelectric focusing, and all fractions had a general formula of C-60(OH)(m)(O)(n) where the values of m and n might vary with their isoelectric points. Results from the capillary electrophoresis studies showed that the negative surface charge densities were different among the fractions of fullerenols, which indicated that the net surface negative charge was determined by the degree of C=O functionalization. In vitro cytotoxicity assays were also performed to determine the effect of purified fullerenols on cytotoxicity and DNA stability in Raw 264.7 cells. Although the fullerenols had low cytotoxicity, the composition of fullerenols with the highest negative charge density (-1.913 +/- 0.008 x 10(-4) C) and smaller size (3.92 +/- 0.71 nm) was found to induce DNA damage. Therefore, crude fullerenols must be separated and purified in order to obtain fullerenols with low degree of C=O functionalization and good biocompatibility. (C) 2011 Elsevier Ltd. All rights reserved. |
学科主题 | Chemistry; Materials Science |
收录类别 | SCI |
WOS记录号 | WOS:000297397700014 |
公开日期 | 2016-05-03 |
内容类型 | 期刊论文 |
源URL | [http://ir.ihep.ac.cn/handle/311005/223988] |
专题 | 高能物理研究所_多学科研究中心 |
推荐引用方式 GB/T 7714 | Li J,Zhang MY,Sun BY,et al. Separation and purification of fullerenols for improved biocompatibility[J]. CARBON,2012,50(2):460-469. |
APA | 李娟.,张铭倚.,孙宝云.,Li, J.,Zhang, MY.,...&常亚男.(2012).Separation and purification of fullerenols for improved biocompatibility.CARBON,50(2),460-469. |
MLA | 李娟,et al."Separation and purification of fullerenols for improved biocompatibility".CARBON 50.2(2012):460-469. |
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