Structural insights into the inhibition of type VI effector Tae3 by its immunity protein Tai3

	Structural insights into the inhibition of type VI effector Tae3 by its immunity protein Tai3
	董程;Dong, C; 高增强;Zhang, H; Gao, ZQ; Wang, WJ; She, Z; Liu, GF; Shen, YQ; Su, XD; Dong, YH; Wang WJ(王文佳); She Z(佘准); Liu GF(刘广峰); Dong YH(董宇辉)
刊名	BIOCHEMICAL JOURNAL
	2013
卷号	454 期号:1 页码:59-68
关键词	NlpC/P60 type VI secretion system (T6SS) type VI amidase effector 3 type VI amidase immunity 3
英文摘要	The recently described T6SS (type VI secretion system) acts as a needle that punctures the membrane of the target cells to deliver effector proteins. Type VI amidase effectors can be classified into four divergent families (Tae1-Tae4). These effectors are secreted into the periplasmic space of neighbouring cells via the T6SS and subsequently rupture peptidoglycan. However, the donor cells are protected from damage because of the presence of their cognate immunity proteins [Tai1 (type VI amidase immunity 1)-Tai4]. In the present paper, we describe the structure of Tae3 in complex with Tai3. The Tae3-Tai3 complex exists as a stable heterohexamer, which is composed of two Tae3 molecules and two Tai3 homodimers (Tae3-Tai3(4)-Tae3). Tae3 shares a common NlpC/P60 fold, which consists of N-terminal and C-terminal subdomains. Structural analysis indicates that two unique loops around the catalytic cleft adopt a closed conformation, resulting in a narrow and extended groove involved in the binding of the substrate. The inhibition of Tae3 is attributed to the insertion of the Omega-loop (loop of alpha 3-alpha 4) of Tai3 into the catalytic groove. Furthermore, a cell viability assay confirmed that a conserved motif (Gln-Asp-Xaa) in Tai3 members may play a key role in the inhibition process. Taken together, the present study has revealed a novel inhibition mechanism and provides insights into the role played by T6SS in interspecific competition.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
WOS记录号	WOS:000322934000006
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/223811]
专题	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	董程；Dong, C,高增强；Zhang, H,Gao, ZQ,et al. Structural insights into the inhibition of type VI effector Tae3 by its immunity protein Tai3[J]. BIOCHEMICAL JOURNAL,2013,454(1):59-68.
APA	董程；Dong, C.,高增强；Zhang, H.,Gao, ZQ.,Wang, WJ.,She, Z.,...&王文佳；佘准；刘广峰；董宇辉.(2013).Structural insights into the inhibition of type VI effector Tae3 by its immunity protein Tai3.BIOCHEMICAL JOURNAL,454(1),59-68.
MLA	董程；Dong, C,et al."Structural insights into the inhibition of type VI effector Tae3 by its immunity protein Tai3".BIOCHEMICAL JOURNAL 454.1(2013):59-68.