Solid-state and biological nanopore for real-time sensing of single chemical and sequencing of DNA

	Solid-state and biological nanopore for real-time sensing of single chemical and sequencing of DNA
	Haque, F; Li, JH; Wu HC(吴海臣); Wu, HC; Liang, XJ; Guo, PX
刊名	NANO TODAY
	2013
卷号	8 期号:1 页码:56-74
关键词	Bacteriophage phi29 alpha-Hemolysin MspA Solid state pore Synthetic nanopores DNA packaging Nanomotor Connector Liposomes Ion channel Single channel conductance Membrane channel Viral assembly Stoichiometry quantification Nanostructure Bionanotechnology Nanobiotechnology Nanomedicine
英文摘要	Sensitivity and specificity are two most important factors to take into account for molecule sensing, chemical detection and disease diagnosis. A perfect sensitivity is to reach the level where a single molecule can be detected. An ideal specificity is to reach the level where the substance can be detected in the presence of many contaminants. The rapidly progressing nanopore technology is approaching this threshold. A wide assortment of biomotors and cellular pores in living organisms perform diverse biological functions. The elegant design of these transportation machineries has inspired the development of single molecule detection based on modulations of the individual current blockage events. The dynamic growth of nanotechnology and nanobiotechnology has stimulated rapid advances in the study of nanopore based instrumentation over the last decade, and inspired great interest in sensing of single molecules including ions, nucleotides, enantiomers, drugs, and polymers such as PEG, RNA, DNA, and polypeptides. This sensing technology has been extended to medical diagnostics and third generation high throughput DNA sequencing. This review covers current nanopore detection platforms including both biological pores and solid state counterparts. Several biological nanopores have been studied over the years, but this review will focus on the three best characterized systems including et-hemolysin and MspA, both containing a smaller channel for the detection of single stranded DNA, as well as bacteriophage phi29 DNA packaging motor connector that contains a larger channel for the passing of double stranded DNA. The advantage and disadvantage of each system are compared; their current and potential applications in nanonnedicine, biotechnology, and nanotechnology are discussed. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Chemistry; Science & Technology - Other Topics; Materials Science
收录类别	SCI
WOS记录号	WOS:000318050600008
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/223807]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Haque, F,Li, JH,Wu HC,et al. Solid-state and biological nanopore for real-time sensing of single chemical and sequencing of DNA[J]. NANO TODAY,2013,8(1):56-74.
APA	Haque, F,Li, JH,吴海臣,Wu, HC,Liang, XJ,&Guo, PX.(2013).Solid-state and biological nanopore for real-time sensing of single chemical and sequencing of DNA.NANO TODAY,8(1),56-74.
MLA	Haque, F,et al."Solid-state and biological nanopore for real-time sensing of single chemical and sequencing of DNA".NANO TODAY 8.1(2013):56-74.