iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies

CORC > 成都山地灾害与环境研究所 > 中国科学院水利部成都山地灾害与环境研究所 > 山地表生过程与生态调控重点实验室

	iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies
	Zhang, Sheng1 ; Zhou, Rong 1,2; Zhao, Hongxia1 ; Korpelainen, Helena 3; Li, Chunyang1,4
刊名	PROTEOMICS
	2016-02-01
卷号	16 期号:4 页码:614-628
关键词	iTRAQ Nitrogen deficiency Phosphorus deficiency Plant proteomics Poplar Sexual differences
ISSN号	1615-9853
通讯作者	Li, Chunyang
英文摘要	Male and female poplars (Populus cathayana Rehd.) respond differently to nitrogen (N) and phosphorus (P) deficiencies. In this study, an iTRAQ-based quantitative proteomic analysis was performed. N and P deficiencies caused 189 and 144 proteins to change in abundance in males and 244 and 464 in females, respectively. Compared to N- and P-deficient males, both N- and P-deficient females showed a wider range of changes in proteins that are involved in amino acid, carbohydrate and protein metabolism, and the sexual differences were significant. When comparing the effects of N- and P-deficiencies, N-deficient females expressed more changes in proteins that are involved in stress responses and gene expression regulation, while P-deficient females showed more changes in proteins that are involved in energy and lipid metabolism, stress responses and gene expression regulation. The quantitative RT-PCR analysis of stress-related proteins showed that males have a better expression correlation between mRNA and protein levels than do females. This study shows that P. cathayana females are more sensitive and have more rapid metabolic mechanisms when responding to N and P deficiencies than do males, and P deficiency has a wider range of effects on females than does N deficiency.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	ALANINE AMINOTRANSFERASE HOMOLOGS ; CARBON-ISOTOPE DISCRIMINATION ; UV-B RADIATION ; POPULUS-CATHAYANA ; DROUGHT STRESS ; USE EFFICIENCY ; PROTECTIVE MECHANISMS ; RNA LIGASE ; WATER-USE ; PLANTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000371150900007
公开日期	2016-04-26
内容类型	期刊论文
源URL	[http://ir.imde.ac.cn/handle/131551/15111]
专题	成都山地灾害与环境研究所_山地表生过程与生态调控重点实验室
作者单位	1.Chinese Acad Sci, Inst Mt Hazards & Environm, Key Lab Mt Surface Proc & Ecol Regulat, Chengdu, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Helsinki, Dept Agr Sci, Viikki Plant Sci Ctr, Helsinki, Finland 4.Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Nurturing Stn, Linan 311300, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Sheng,Zhou, Rong,Zhao, Hongxia,et al. iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies[J]. PROTEOMICS,2016,16(4):614-628.
APA	Zhang, Sheng,Zhou, Rong,Zhao, Hongxia,Korpelainen, Helena,&Li, Chunyang.(2016).iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies.PROTEOMICS,16(4),614-628.
MLA	Zhang, Sheng,et al."iTRAQ-based quantitative proteomic analysis gives insight into sexually different metabolic processes of poplars under nitrogen and phosphorus deficiencies".PROTEOMICS 16.4(2016):614-628.