Highly soluble and stable recombinant holo-phycocyanin alpha subunit expressed in Escherichia coli | |
Liu, Shaofang1,2; Chen, Huaxin1; Qin, Song1![]() | |
刊名 | BIOCHEMICAL ENGINEERING JOURNAL
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2009-12-15 | |
卷号 | 48期号:1页码:58-64 |
关键词 | Maltose-binding protein Phycocyanin-alpha subunit Protein engineering Recombinant protein Chromatography Recombinant protein production |
英文摘要 | C-phycocyanin (Cpc) is one of the phycobiliproteins with highly fluorescent and various pharmacological activities Holo-Cpc-alpha Subunit (holo-CpcA) expressed in Escherichia colt resulted in low yield and tended to aggregate after purification in this Study, we constructed a new plasmid coding holo-CpcA fused with hexahistidine and maltose-binding protein tag, which designated as HMCpcA. to Improve Its Solubility and stability without the Impairment of its spectra anti fluorescent properties HMCpcA was significantly more stable over time and a wider range of pH as compared to holo-CpcA. In addition. both the solubility and yields of HMCpcA increase significantly We here provided an example to demonstrate that MBP could also Improve the stability of the protein it fused while it has been reported as a soluble fusion partner before. This novel fluorescent protein will facilitate the large-scale production and be potentially applicable for the development Of fluorescent probes, as well as antioxidant agents (C) 2009 Elsevier B V. All rights reserved |
研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
关键词[WOS] | MALTOSE-BINDING PROTEIN ; C-PHYCOCYANIN ; CYANOBACTERIAL PHYCOBILISOMES ; INVITRO ATTACHMENT ; HETEROLOGOUS HOST ; FUSION PROTEINS ; AFFINITY TAGS ; BIOSYNTHESIS ; ALLOPHYCOCYANIN ; ANTIOXIDANT |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000274349900009 |
公开日期 | 2016-04-26 |
内容类型 | 期刊论文 |
源URL | [http://ir.yic.ac.cn/handle/133337/9227] ![]() |
专题 | 烟台海岸带研究所_中科院烟台海岸带研究所知识产出 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.China Univ Geosci, Sch Marine Sci, Beijing 100083, Peoples R China 4.Chinese Acad Sci, Yantai Inst Costal Zone Res Sustainable Dev, Yantai 264003, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Shaofang,Chen, Huaxin,Qin, Song,et al. Highly soluble and stable recombinant holo-phycocyanin alpha subunit expressed in Escherichia coli[J]. BIOCHEMICAL ENGINEERING JOURNAL,2009,48(1):58-64. |
APA | Liu, Shaofang,Chen, Huaxin,Qin, Song,Zhang, Weijie,Guan, Xiangyu,&Lu, Yandu.(2009).Highly soluble and stable recombinant holo-phycocyanin alpha subunit expressed in Escherichia coli.BIOCHEMICAL ENGINEERING JOURNAL,48(1),58-64. |
MLA | Liu, Shaofang,et al."Highly soluble and stable recombinant holo-phycocyanin alpha subunit expressed in Escherichia coli".BIOCHEMICAL ENGINEERING JOURNAL 48.1(2009):58-64. |
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