Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses

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	Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses
	Zhang, Chao 1,2; Li, Zhouquan 1,2; Zhang, Xiaohua 1; Yuan, Li 1; Dai, Heping 1; Xiao, Wei 3,4
刊名	YEAST
	2016
卷号	33 期号:1 页码:5-19
关键词	Saccharomyces cerevisiae RNA-seq chemical exposure risk assessment YAP1
ISSN号	0749-503X
英文摘要	Transcriptomic profiles are generated by comparing wild-type and the yeast yap1 mutant to various chemicals in an attempt to establish a correlation between this gene mutation and chemical exposure. Test chemicals include ClonNAT as a non-genotoxic agent, methyl methanesulphonate (MMS) as an alkylating agent, tert-butyl hydroperoxide (t-BHP) as an oxidative agent and the mixture of t-BHP and MMS to reflect complex natural exposure. Differentially expressed genes (DEGs) were identified and specific DEGs were obtained by excluding overlapping DEGs with the control group. In the MMS exposure group, deoxyribonucleotide biosynthetic processes were upregulated, while oxidation-reduction processes were downregulated. In the t-BHP exposure group, metabolic processes were upregulated while peroxisome and ion transport pathways were downregulated. In the mixture exposure group, the proteasome pathway was upregulated, while the aerobic respiration was downregulated. Homologue analysis of DEGs related to human diseases showed that many of DEGs were linked to cancer, ageing and neuronal degeneration. These observations confirm that the yap1 mutant is more sensitive to chemicals than wild-type cells and that the susceptible individuals carrying the YAP1-like gene defect may enhance risk to chemical exposure. Hence, this study offers a novel approach to environmental risk assessment, based on the genetic backgrounds of susceptible individuals. Copyright (c) 2015 John Wiley & Sons, Ltd.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
关键词[WOS]	GENOTOXICITY TESTING SYSTEM ; DNA-DAMAGING AGENTS ; RNA-SEQ DATA ; SACCHAROMYCES-CEREVISIAE ; GENE-EXPRESSION ; ENHANCES SENSITIVITY ; ENVIRONMENTAL-HEALTH ; TOXICOGENOMICS ; TOXICOLOGY ; NETWORKS
收录类别	SCI
语种	英语
WOS记录号	WOS:000367950400002
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27592]
专题	水生生物研究所_水环境工程研究中心
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China 4.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK, Canada
推荐引用方式 GB/T 7714	Zhang, Chao,Li, Zhouquan,Zhang, Xiaohua,et al. Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses[J]. YEAST,2016,33(1):5-19.
APA	Zhang, Chao,Li, Zhouquan,Zhang, Xiaohua,Yuan, Li,Dai, Heping,&Xiao, Wei.(2016).Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses.YEAST,33(1),5-19.
MLA	Zhang, Chao,et al."Transcriptomic profiling of chemical exposure reveals roles of Yap1 in protecting yeast cells from oxidative and other types of stresses".YEAST 33.1(2016):5-19.