Use of a New Enzyme Extraction System to Improve the Sensitivity of SOS/umu Test and Application to Environmental Samples

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	Use of a New Enzyme Extraction System to Improve the Sensitivity of SOS/umu Test and Application to Environmental Samples
	Tian, Zhe; Oda, Yoshimitsu; Zhang, Yu; Yang, Min; Li, Hongyan
刊名	BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
	2015-03
卷号	94 期号:3 页码:370-375
关键词	SOS/umu test beta-Galactosidase activity BugBuster Master Mix Sodium dodecyl sulfate treatment Genotoxicity
英文摘要	The purpose of this study was to find a better enzyme extraction reagent for the SOS/umu test to replace the conventional one (the combination of sodium dodecyl sulfate (SDS) and Z-buffer), which has the disadvantage of denaturing beta-galactosidase leading to decreased measurement sensitivity. By adopting a microplate system, the performance of the umu test using BugBuster Master Mix, a commercially available enzyme extraction reagent, was compared with that using the conventional reagent for detecting the genotoxicity of known mutagens as well as environmental samples. BugBuster Master Mix was found to increase the detection sensitivities of the selected genotoxins and environmental water samples, due to the fact that it doesn't denature beta-galactosidase. The result of this study showed that BugBuster Master Mix could be a better enzyme extraction reagent for umu test.
研究领域[WOS]	Environmental Sciences ; Toxicology
WOS记录号	WOS:000349960900018
公开日期	2016-03-15
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/32869]
专题	生态环境研究中心_环境水质学国家重点实验室
推荐引用方式 GB/T 7714	Tian, Zhe,Oda, Yoshimitsu,Zhang, Yu,et al. Use of a New Enzyme Extraction System to Improve the Sensitivity of SOS/umu Test and Application to Environmental Samples[J]. BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,2015,94(3):370-375.
APA	Tian, Zhe,Oda, Yoshimitsu,Zhang, Yu,Yang, Min,&Li, Hongyan.(2015).Use of a New Enzyme Extraction System to Improve the Sensitivity of SOS/umu Test and Application to Environmental Samples.BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,94(3),370-375.
MLA	Tian, Zhe,et al."Use of a New Enzyme Extraction System to Improve the Sensitivity of SOS/umu Test and Application to Environmental Samples".BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 94.3(2015):370-375.