Investigation on the toxic interaction of superparamagnetic iron oxide nanoparticles with catalase

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Investigation on the toxic interaction of superparamagnetic iron oxide nanoparticles with catalase
	Yu, Zehua; Liu, Hongwei; Hu, Xinxin; Song, Wei; Liu, Rutao
刊名	JOURNAL OF LUMINESCENCE
	2015-03
卷号	159 页码:312-316
关键词	Nanotoxicology Superparamagnetic nanometer iron oxide Catalase Molecular conformation Function
英文摘要	Superparamagnetic iron oxide nanoparticles (SPIONs) have been investigated for various applications in targeted drug delivery and magnetic resonance imaging. Given their clinical relevance, there is a need to understand these particles' potential cytotoxic effects and possible mechanisms of cytotoxicity. Using a variety of spectroscopic techniques, we investigated the interaction of SPIONs with catalase (CAT) in an aqueous environment. Catalase is an important enzyme that protects cells and tissues from oxidative damage by reactive oxygen species (ROS). Therefore, in this work, CAT served as a model protein for examining the physiological effects of SPIONs due to is function in eliminating H2O2. Synchronous fluorescence spectroscopy results showed that SPIONs have little effect on tryptophan residues in CAT. Data from circular dichroism (CD) and UV-vis spectroscopies showed that CAT a-helical content decreased from 32.4% to 29.1% in the presence of SPIONs. Moreover, a ca. 10% decrease in CAT activity was observed in the presence of SPIONs at a 20:1 particle:protein ratio. These results show that SPIONs can interact with proteins to alter both their structure and function. Further studies with CAT or other toxicologically relevant enzymes may be used for elucidating the mechanisms of SPION cytotoxicity. (C) 2014 Elsevier B.V. All rights reserved.
研究领域[WOS]	Optics
WOS记录号	WOS:000348953800043
公开日期	2016-03-15
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/32607]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Yu, Zehua,Liu, Hongwei,Hu, Xinxin,et al. Investigation on the toxic interaction of superparamagnetic iron oxide nanoparticles with catalase[J]. JOURNAL OF LUMINESCENCE,2015,159:312-316.
APA	Yu, Zehua,Liu, Hongwei,Hu, Xinxin,Song, Wei,&Liu, Rutao.(2015).Investigation on the toxic interaction of superparamagnetic iron oxide nanoparticles with catalase.JOURNAL OF LUMINESCENCE,159,312-316.
MLA	Yu, Zehua,et al."Investigation on the toxic interaction of superparamagnetic iron oxide nanoparticles with catalase".JOURNAL OF LUMINESCENCE 159(2015):312-316.