Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC

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	Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC
	Zeng, HL; Liu, Q; Yu, JG; Wang, ML; Chen, M; Wang, RH; He, X; Gao, MH; Chen, XQ
刊名	JOURNAL OF SEPARATION SCIENCE
	2015
卷号	38 期号:22 页码:3897—3904
关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY IMMOBILIZED ENZYME RECYCLING ELUTION IN-VITRO PURIFICATION LEAVES PHASE POLYSACCHARIDE IDENTIFICATION OPTIMIZATION
ISSN号	1615-9306
通讯作者	Chen, XQ (reprint author), Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
英文摘要	In this study, an on-line two-dimensional high-speed counter-current chromatography system based on a six-port valve was developed. Target-guided by ultrafiltration with high-performance liquid chromatography, the one-step isolation of three potential alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench was achieved by employing the developed orthogonal system and extrusion elution mode. The purities of three potential alpha-amylase inhibitors were all over 95% as determined by high-performance liquid chromatography. Furthermore, UV, mass spectrometry and H-1 NMR spectroscopy were applied to the structural identification of the isolated three target compounds, their structures were assigned as quercetin-3-O-sophoroside (i), 5,7,3',4'-tetrahydroxy flavonol-3-O-[beta-D-rhamnopyranosil(1 -> 2)]-beta-D-glucopyranoside (ii) and isoquercitrin (iii), respectively. The Results demonstrated that the proposed method was highly efficient to screen and isolate enzyme inhibitors from complex natural products extracts, and on-line two-dimensional high-speed countercurrent chromatography can effectively increase the peak resolution of target compounds.
语种	英语
WOS记录号	WOS:000365320800012
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/25036]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Zeng, HL,Liu, Q,Yu, JG,et al. Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(22):3897—3904.
APA	Zeng, HL.,Liu, Q.,Yu, JG.,Wang, ML.,Chen, M.,...&Chen, XQ.(2015).Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC.JOURNAL OF SEPARATION SCIENCE,38(22),3897—3904.
MLA	Zeng, HL,et al."Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC".JOURNAL OF SEPARATION SCIENCE 38.22(2015):3897—3904.