Optimization of ultrasound-assisted extraction parameters of chlorophyll from Chlorella vulgaris residue after lipid separation using response surface methodology

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	Optimization of ultrasound-assisted extraction parameters of chlorophyll from Chlorella vulgaris residue after lipid separation using response surface methodology
	Kong WB(孔维宝)1,2; Liu, Na 1; Zhang, Ji 1; Yang, Qi 1; Hua SF(华绍烽)2 ; Song H(宋昊)2 ; Xia CG(夏春谷)2
刊名	Journal of Food Science and Technology
	2014
卷号	51 期号:9 页码:2006-2013
关键词	Chlorophyll Chlorella vulgaris Ultrasound-assisted extraction Response surface methodology Optimization
ISSN号	0022-1155
通讯作者	孔维宝 ; 夏春谷
英文摘要	An investigation into ultrasound-assisted extraction (UAE) was conducted for the extraction of chlorophyll from Chlorella vulgaris residue after lipid separation. The best possible combination of extraction parameters was obtained with the response surface methodology (RSM), at a three-variable, three-level experiment Box–Behnken design (BBD). The optimum extraction parameters were as follows: extraction temperature, 61.4 °C, extraction time, 78.7 min, ethanol volume, 79.4 %, at a fixed ultrasonic power of 200 W. Under the modified optimal conditions, the model predicted a total chlorophyll content of 30.1 mg/g. Verification of the optimization showed that chlorophyll extraction of 31.1 ± 1.56 mg/g was observed under the optimal conditions, which well matches with the predicted value. Under these conditions, two stage extraction could sufficiently reach the maximal chlorophyll yield (35.2 mg/g), and the extraction rate reached up to 88.9 %. The present paper provides a feasible technology route for comprehensive utilization of bioactive substances from Chlorella and microalgal biomass biorefinery.
学科主题	物理化学与绿色催化
收录类别	SCI
资助信息	National Science Fund for Distinguished Young Scholars of China (Grant No. 20625308);Research Fund for Young Teachers of Northwest Normal University (Grant No. NWNU-LKQN-10-30)
语种	英语
WOS记录号	WOS:000341701700036
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/19235]
专题	兰州化学物理研究所_OSSO国家重点实验室
作者单位	1.Northwest Normal Univ, Coll Life Sci, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, State Key Lab Oxo Synth & Select Oxidat, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Kong WB,Liu, Na,Zhang, Ji,et al. Optimization of ultrasound-assisted extraction parameters of chlorophyll from Chlorella vulgaris residue after lipid separation using response surface methodology[J]. Journal of Food Science and Technology,2014,51(9):2006-2013.
APA	Kong WB.,Liu, Na.,Zhang, Ji.,Yang, Qi.,Hua SF.,...&Xia CG.(2014).Optimization of ultrasound-assisted extraction parameters of chlorophyll from Chlorella vulgaris residue after lipid separation using response surface methodology.Journal of Food Science and Technology,51(9),2006-2013.
MLA	Kong WB,et al."Optimization of ultrasound-assisted extraction parameters of chlorophyll from Chlorella vulgaris residue after lipid separation using response surface methodology".Journal of Food Science and Technology 51.9(2014):2006-2013.