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	Arsenite, arsenate and vanadate affect human erythrocyte membrane
	Zhang, TL; Gao, YX; Lu, JF; Wang, K
	2000
会议名称	5th International Symposium on Applied Bioinorganic Chemistry (5 ISABC)
会议日期	APR 13-17, 1999
会议地点	CORFU, GREECE
关键词	arsenite arsenate vanadate human erythrocyte membrane proteins
英文摘要	Effects of arsenite, arsenate and vanadate on human erythrocyte membrane have been assessed according to their routes passing through the membrane, their binding modes to the membrane and their influences on membrane proteins and lipids. The uptake of arsenate (1.0 mM) by cells approached a limit with intracellular arsenic of about 0.2 mM in 5 h, and was strongly inhibited (similar to 95 %) by 4,4'-diisothiocyano-2,2'-disulfonic stilbene (DIDS), indicating that arsenate, similar to vanadate, passed across the membrane through the anion exchange protein, band 3. Arsenite (1.0 mM) influx reached a maximum of about 0.4 mM in 30 min, and was not inhibited by DIDS. The transformed species of arsenite bound to the membrane from cytosol. In contrast, arsenate bound rapidly from the outside, followed by releasing and re-binding. The binding to the membrane via sulfhydryl was indicated by the decrease of the sulfhydryl level of membrane proteins. Polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS-PAGE) analysis revealed that the proteins, bands 1-3, were among the targets of arsenite, arsenate and vanadate. Their binding to the membrane also induced changes in the fluidity of membrane lipids and in the negative charge density in the outer surface of the membrane. (C) 2000 Elsevier Science Inc. All rights reserved.
ISSN号	0162-0134
内容类型	会议论文
源URL	[http://ir.rcees.ac.cn/handle/311016/24844]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhang, TL,Gao, YX,Lu, JF,et al. Arsenite, arsenate and vanadate affect human erythrocyte membrane[C]. 见:5th International Symposium on Applied Bioinorganic Chemistry (5 ISABC). CORFU, GREECE. APR 13-17, 1999.

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