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	Optimizing the quadruple-potential waveform for the pulsed amperometric detection of neomycin
	Ding, YS; Yu, H; Mou, SF
	2004
会议名称	16th International Ion Chromography Symposium
会议日期	SEP 21-24, 2003
会议地点	San Diego, CA
关键词	disinfectants benzyldimethyldodecylammonium bromide benzalkonium bromide quaternary ammonium compounds surfactants
英文摘要	Two novel analytical methodologies using capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) were developed and compared for the determination of benzyldimethyldodecylammonium bromide (BAB) in commercial compound chemical disinfectants. The LC analysis was performed witha Kromasil C-18 (200 mm x 4.6 mm, 5 mum) column and a mobile phase of A:B = 80:20 (A: acetonitrile, B: 4 mmol/L octanesulfonic sodium-0.02 mol/L acetic sodium, adjusted with acetic acid to pH 5.2) at a flow rate of 1.0 mL/min. Detection was by ultraviolet absorption at 262 nm. The CE analysis was performed in a bare fused-silica capillary with 75 mum W. and total length of 46.4 cm with a buffer solution of 50% acetonitrile -50 mmol/L NaH2,PO4, pH 2.24. The applied voltage was 20 kV. Detection was by ultraviolet absorption at 214 run. Under optimized conditions, the HPLC retention time and CE migration time for BAB was 9.18 and 5.08 min, respectively. Calibration curves of peak area versus concentration gave correlation coefficients of 0.9996 for HPLC and 0.9994 for CE. The detection limits for HPLC and CE were 1.6 mg/L and 0.2 mg/L, respectively. Average recoveries at three concentration levels (50, 100, 200 mg/L for HPLC; 20, 40, 100 mg/L for CE) were 99.94 +/- 1.5, 99.64 +/- 1.3 and 99.61 +/- 0.4% for HPLC and 120.47 +/- 2.6, 102.06 +/- 8.7 and 103.05 +/- 3.0% for CE, respectively. Although both methods were shown to be suitable for the determination of BAB in commercial disinfectant compounds, CE provided analysis with less solvent purchase/disposal and better column efficiency, whereas HPLC provided superior precision. (C) 2004 Elsevier B.V. All rights reserved.
内容类型	会议论文
源URL	[http://ir.rcees.ac.cn/handle/311016/24783]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Ding, YS,Yu, H,Mou, SF. Optimizing the quadruple-potential waveform for the pulsed amperometric detection of neomycin[C]. 见:16th International Ion Chromography Symposium. San Diego, CA. SEP 21-24, 2003.

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