QQ客服 官方微博 反馈留言

	Determination of estrogens and bisphenol A in bovine milk by automated on-line C-30 solid-phase extraction coupled with high-performance liquid chromatography-mass spectrometry
	Yan, Wei; Li, Ying; Zhao, Lixia; Lin, Jin-Ming
	2009
会议名称	33rd International Symposium on High Performance Liquid Phase Separations and Related Techniques
会议日期	DEC 02-05, 2008
会议地点	Kyoto, JAPAN
关键词	Triacontyl bonded silica Estrogens Liquid chromatography mass spectrometry Spiked milk samples
英文摘要	Using triacontyl bonded silica (C-10) as on-line solid-phase extraction (SPE) material and a specially designed on-line analytical system which allowed large sample volume injection, a high speed and robust on-line SPE-HPLC-MS method was established for I tie analysis of five estrogens and bisphenol A(BPA)m milk samples The milk sample is pretreated with acetonitrile for protein precipitation and then treated with primary secondary amine for the removal of Polar impurities tries in the matrix Then the pretreated sample can be automatically loaded by a LC pump. For effective extraction. an offshoot wit 11 NH4Ac solution of high-flow rate was employed dilute the loaded sample by a mixing tee before sample was loaded onto the C-30 extraction column. In this way. large volume injection (1 mL in this experiment)could be achieved Some Important parameters such as sample loading flow rate, sample dilution ratio and injection volume were optimized Under the optimized conditions, the recoveries for all analytes range from 714 to 97 1% and reproducibility represented as RSDs are less than 15 0% (n = 5) with milk samples spiked at 0 6 and 15 ng/mL. of each analyte To the authors' knowledge. It Constitutes the first work describing a C-30 on-line SPE-LC-MS analytical method for the screen and monitoring of these estrogens and BPA in milk (C) 2009 Elsevier B.V. rights reserved
ISSN号	0021-9673
内容类型	会议论文
源URL	[http://ir.rcees.ac.cn/handle/311016/24672]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Yan, Wei,Li, Ying,Zhao, Lixia,et al. Determination of estrogens and bisphenol A in bovine milk by automated on-line C-30 solid-phase extraction coupled with high-performance liquid chromatography-mass spectrometry[C]. 见:33rd International Symposium on High Performance Liquid Phase Separations and Related Techniques. Kyoto, JAPAN. DEC 02-05, 2008.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。