Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories
Luan, Guodong1,2; Qi, Yunjing4; Wang, Min1,3; Li, Zhimin1,2; Duan, Yangkai1,3,5; Tan, Xiaoming1,2; Lu, Xuefeng1,2
刊名BIOTECHNOLOGY FOR BIOFUELS
2015-11-21
卷号8
关键词In vitro reconstitution Steady-state analysis Ethanol Cyanobacteria Metabolic engineering
英文摘要Background: Photosynthetic production of chemicals and fuels by recycling CO2 in cyanobacteria is a promising solution facing energy shortage and resource declination. Ethanol is an attractive and demonstrative biofuel product, and ethanol synthesis in cyanobacteria has been achieved by assembling of a pathway consisting of pyruvate decarboxylase (PDCzm) and alcohol dehydrogenase II (slr1192). For enabling more powerful ethanol photosynthetic production, an optimized and balanced catalyzing route was required. In this work, we provided a paradigm for systematically characterizing and optimizing the PDCzm-slr1192 pathway from engineered cyanobacteria strains, combining in vitro reconstitution, genetic engineering and feeding-cultivation.
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]SYNECHOCYSTIS SP PCC6803 ; SP PCC 6803 ; IN-VITRO RECONSTITUTION ; FATTY-ACID SYNTHASE ; CARBON-DIOXIDE ; PHOTOSYNTHETIC PRODUCTION ; ESCHERICHIA-COLI ; LACTIC-ACID ; ZYMOMONAS-MOBILIS ; 2,3-BUTANEDIOL
收录类别SCI
语种英语
WOS记录号WOS:000365783700001
公开日期2016-02-26
内容类型期刊论文
源URL[http://ir.qibebt.ac.cn/handle/337004/6800]  
专题青岛生物能源与过程研究所_微生物代谢工程团队
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China
4.Qingdao Univ Sci & Technol, Qingdao 266061, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Luan, Guodong,Qi, Yunjing,Wang, Min,et al. Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories[J]. BIOTECHNOLOGY FOR BIOFUELS,2015,8.
APA Luan, Guodong.,Qi, Yunjing.,Wang, Min.,Li, Zhimin.,Duan, Yangkai.,...&Lu, Xuefeng.(2015).Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories.BIOTECHNOLOGY FOR BIOFUELS,8.
MLA Luan, Guodong,et al."Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories".BIOTECHNOLOGY FOR BIOFUELS 8(2015).
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