characterization of a new bradykinin potentiating peptide from trimeresurus mucrosquamatus

	characterization of a new bradykinin potentiating peptide from trimeresurus mucrosquamatus
	JIA Yong-Hong ; LI Dong-Sheng ; ZHU Shao-Wen ; Zhang Li-Yue ; Ding Li-Sheng ; WANG Wan-Yu ; XIONG Yu-Liang
刊名	生物化学与生物物理学报
	2003
卷号	35 期号:7 页码:619-623
关键词	angiotensin-converting enzyme bradykinin bradykinin-potentiating peptide
通讯作者	XIONG Yu-Liang
产权排序	3
合作状况	其它
中文摘要	A novel bradykinin-potentiating peptide (BPP), designated as TmF, has been purified to homogeneity from the venom of Trimeresurus mucrosquamatus by 70% cold methanol extraction, Sephadex G-15 gel filtration and reverse-phase high performance liquid chromatography (RP-HPLC). The amino acid sequence of TmF was determined to be pGlu-Gly-Arg-Pro-Leu-Gly-Pro-Pro-Ile-Pro-Pro (pGlu denotes pyroglutamic acid), which shared high homology with other BPPs. The molecular mass of TmF was 1.1107 kD as determinated by electrospray ionization-mass spectrometry (ESI-MS), which was in accordance with the calculated value of 1.1106 kD. The potentiating unit of TmF to bradykinin-induced (BK-induced) contraction on the guinea-pig ileum in vitro was (1.13 +/-0.3) unit (mg/L), and TmF (5.0 x10(-4) mg/kg) increased the pressure-lowering-effect of bradykinin (5.0 x10(-5 )mg/kg) with approximate descent value of (14 +/-2) mmHg. In addition, TmF inhibited the conversion of angiotensin I to angiotensin II, 2 x10(-3) mg of TmF caused 50% inhibition (IC(50)) of angiotensin- converting enzyme (ACE) hydrolyzing activity to bradykinin.
学科主题	天然产物研究
收录类别	SCI
语种	英语
公开日期	2016-02-26
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/26735]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	JIA Yong-Hong,LI Dong-Sheng,ZHU Shao-Wen,et al. characterization of a new bradykinin potentiating peptide from trimeresurus mucrosquamatus[J]. 生物化学与生物物理学报,2003,35(7):619-623.
APA	JIA Yong-Hong.,LI Dong-Sheng.,ZHU Shao-Wen.,Zhang Li-Yue.,Ding Li-Sheng.,...&XIONG Yu-Liang.(2003).characterization of a new bradykinin potentiating peptide from trimeresurus mucrosquamatus.生物化学与生物物理学报,35(7),619-623.
MLA	JIA Yong-Hong,et al."characterization of a new bradykinin potentiating peptide from trimeresurus mucrosquamatus".生物化学与生物物理学报 35.7(2003):619-623.