Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe

	Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe
	Long, Jun ; Wang, Xing-ming ; Xu, Dong-ling ; Ding, Li-sheng
刊名	JOURNAL OF MOLECULAR RECOGNITION
	2014
卷号	27 期号:3 页码:131-137
关键词	safranin T fluorescence spectra DNA interaction acridine orange
通讯作者	Wang, XM (reprint author), Southwest Univ Sci & Technol, Sch Mat Sci & Engn, Mianyang 621010, Peoples R China.
产权排序	2
合作状况	其它
英文摘要	Under the condition of physiological pH environment (pH=7.40), the interactions of safranin T (ST) with herring sperm DNA were studied by means of spectral methods using acridine orange (AO) as a fluorescence probe. The spectroscopic characteristics of DNA-AO in the case of ST (along with the increase of concentration) were observed in an aqueous medium. The binding constants for ST stranded DNA and competitive bindings of ST interacting with DNA-AO systems were examined by fluorescence spectra, and the binding mechanism of ST with DNA was researched via viscosity measurements. All the testimony manifested that bonding modes between ST and DNA were evidenced to be intercalative binding and electrostatic binding, and the combining constant of ST with DNA was obtained. The binding of ST to DNA was driven by entropy and enthalpy through the calculated thermodynamic parameters (H-r(m)?, S-r(m) and (r)G(m)(?)). Copyright (c) 2014 John Wiley & Sons, Ltd.
学科主题	Biochemistry & Molecular Biology; Biophysics
收录类别	SCI
语种	英语
公开日期	2016-02-26
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/26696]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Long, Jun,Wang, Xing-ming,Xu, Dong-ling,et al. Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe[J]. JOURNAL OF MOLECULAR RECOGNITION,2014,27(3):131-137.
APA	Long, Jun,Wang, Xing-ming,Xu, Dong-ling,&Ding, Li-sheng.(2014).Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe.JOURNAL OF MOLECULAR RECOGNITION,27(3),131-137.
MLA	Long, Jun,et al."Spectroscopic studies on the interaction mechanisms of safranin T with herring sperm DNA using acridine orange as a fluorescence probe".JOURNAL OF MOLECULAR RECOGNITION 27.3(2014):131-137.