HBx affects CUL4-DDB1 function in both positive and negative manners

	HBx affects CUL4-DDB1 function in both positive and negative manners
	Guo, Liandi ; Wang, Xiaobo ; Ren, Laifeng ; Zeng, Ming ; Wang, Si ; Weng, Yuding ; Tang, Zizhi ; Wang, Xin ; Tang, Yaxiong ; Hu, Huaidong ; Li, Mingyuan ; Zhang, Chongjie ; Liu, Cong
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2014
卷号	450 期号:4 页码:1492-1497
关键词	Hepatitis B virus HBx DDB1 cccDNA PRMT1
通讯作者	Li, MY (reprint author), Sichuan Univ, West China Sch Preclin & Forens Med, Dept Microbiol, Chengdu 610041, Sichuan, Peoples R China.
产权排序	4
合作状况	其它
英文摘要	Hepatitis B virus (HBV) infection is a major public health problem by affecting 350 million people worldwide. The mechanisms that regulate HBV gene expression and viral replication remain poorly understood. HBx is known as the central regulator for HBV replication and is associated with the CUL4-DDB1 ubiquitin ligase through H-box motif. Here, we show that blocking the activity of DDB1 by RNA interfering inhibited viral production and gene expression of HBV, and direct association of HBx with DDB1 promoted viral activities, indicating that DDB1 function is required for viral production. On the other hand, HBx interfered with DDB1-dependent polyubiquitination of PRMT1, arginine methyltransferase 1, suggesting that HBx can also block the function of a subset of CUL4-DDB1 E3 ligases. Thus, we conclude that HBx regulates the function of DDB1 in both positive and negative manners in the context of distinct CUL4-DDB1 complexes and plays different roles in HBV replication cycle. (C) 2014 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
收录类别	SCI
语种	英语
公开日期	2016-02-26
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/26672]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Guo, Liandi,Wang, Xiaobo,Ren, Laifeng,et al. HBx affects CUL4-DDB1 function in both positive and negative manners[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,450(4):1492-1497.
APA	Guo, Liandi.,Wang, Xiaobo.,Ren, Laifeng.,Zeng, Ming.,Wang, Si.,...&Liu, Cong.(2014).HBx affects CUL4-DDB1 function in both positive and negative manners.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,450(4),1492-1497.
MLA	Guo, Liandi,et al."HBx affects CUL4-DDB1 function in both positive and negative manners".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 450.4(2014):1492-1497.