Simultaneous Quantification of Salvianolic Acid B and Tanshinone IIA of Salvia Tropolone Tablets by UPLC-MRM-MS/MS for Pharmacokinetic Studies

	Simultaneous Quantification of Salvianolic Acid B and Tanshinone IIA of Salvia Tropolone Tablets by UPLC-MRM-MS/MS for Pharmacokinetic Studies
	Li, J. R. ; Li, D. X. ; Li, L. ; Deng, W. L. ; Ding, L. S. ; Xu, H. X. ; Zhou, Y.
刊名	ACTA CHROMATOGRAPHICA
	2014
卷号	26 期号:4 页码:711-725
关键词	salvia tropolone tablets tanshinone IIA salvianolic acid B UPLC-MRM-MS/MS pharmacokinetic study
通讯作者	Zhou, Y (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China.
产权排序	1
合作状况	其它
英文摘要	A rapid and sensitive ultraperformance liquid chromatography-multiple reaction monitoring-multi-stage/mass spectrometry (UPLC-MRM-MS/MS) method has been developed for simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets in dog plasma. This was achieved by performing quantification using the MRM acquisition with two channels of MRM-MS/MS and MS full scan for more accuracy qualitative results, and the fragmentation transitions of m/z 295 -> 249, 191 for tanshinone IIA and m/z 297 -> 279, 251 for IS in positive mode, m/z 717 -> 519, 321 for salvianolic acid B and m/z 295 -> 267, 239 for IS in negative mode were selected. The UPLC separation was achieved within 3 min in a single UPLC run. Linear calibration curves were obtained over the concentration range of 10 pg/mL(-1) ng/mL for tanshinone IIA and 100 pg/mL(-1) for salvianolic acid B. Lower limit of quantitation (LLOQ) was 10 pg/mL and 100 pg/mL for tanshinone IIA and salvianolic acid B, respectively. The inter-day and intra-day precision (relative standard deviation, RSD) in all samples were less than 8.21%, and the recoveries were over 85.9% for both tanshinone IIA and salvianolic acid B. The two channels of MRM with MS full scan approach could provide both qualitative and quantitative results without the need for repetitive analyses and resulted in the reduction of further confirmation experiments and analytical time. The pharmacokinetic study of the two active components of salvia tropolone tablets following oral gavage administration of dogs was thus explored with this method.
学科主题	Chemistry, Analytical
收录类别	SCI
语种	英语
公开日期	2016-02-26
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/26655]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Li, J. R.,Li, D. X.,Li, L.,et al. Simultaneous Quantification of Salvianolic Acid B and Tanshinone IIA of Salvia Tropolone Tablets by UPLC-MRM-MS/MS for Pharmacokinetic Studies[J]. ACTA CHROMATOGRAPHICA,2014,26(4):711-725.
APA	Li, J. R..,Li, D. X..,Li, L..,Deng, W. L..,Ding, L. S..,...&Zhou, Y..(2014).Simultaneous Quantification of Salvianolic Acid B and Tanshinone IIA of Salvia Tropolone Tablets by UPLC-MRM-MS/MS for Pharmacokinetic Studies.ACTA CHROMATOGRAPHICA,26(4),711-725.
MLA	Li, J. R.,et al."Simultaneous Quantification of Salvianolic Acid B and Tanshinone IIA of Salvia Tropolone Tablets by UPLC-MRM-MS/MS for Pharmacokinetic Studies".ACTA CHROMATOGRAPHICA 26.4(2014):711-725.